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a Oregon Regional Primate Research Center, Beaverton, Oregon 97006
b Department of Obstetrics and Gynecology, and Cell and Developmental Biology, Oregon Health Sciences University, Beaverton, Oregon 97006
c WM Keck Autoimmune Disease Center, The Scripps Research Institute, La Jolla, California 92037
d Reproduction and Developmental Biology Unit, Faculty of Biological Sciences, Pontifical Catholic University of Chile, Santiago, Chile
e Center for Neuroscience and Cell Biology of Coimbra, Department of Zoology, University of Coimbra, Coimbra, Portugal
ABSTRACT
Vesicular membrane trafficking during acrosome biogenesis in bull and rhesus monkey spermatogenesis differs from the somatic cell paradigm as imaged dynamically using the Golgi apparatus probes ß-COP, giantin, Golgin-97, and Golgin-95/GM130. In particular, sorting and delivery of proteins seemed less precise during spermatogenesis. In early stages of spermiogenesis, many Golgi resident proteins and specific acrosomal markers were present in the acrosome. Trafficking in both round and elongating spermatids was similar to what has been described for somatic cells, as judged by the kinetics of Golgi protein incorporation into endoplasmic reticulum-like structures after brefeldin A treatment. These Golgi components were retrieved from the acrosome at later stages of differentiation and were completely devoid of immature spermatozoa. Our data suggest that active anterograde and retrograde vesicular transport trafficking pathways, involving both ß-COP- and clathrin-coated vesicles, are involved in retrieving Golgi proteins missorted to the acrosome and in controlling the growth and shape of this organelle.
First decision: 21 January 2000.
1 The Mellon Foundation sponsored R.M. J.R.S. is a recipient of a Praxis XXI postdoctoral fellowship from Fundação para a Ciência e Tecnologia (FCT, Portugal). This research was sponsored by research grants from the NIH (NICHD, NCRR) to G.S and by NICHD/NIH through cooperative agreement [U54 18185] as part of the Specialized Cooperative Centers Program in Reproduction Research. The ORPRC infrastructure is sponsored as an NCRR Regional Primate Research Center.
2 Correspondence: Gerald Schatten, Oregon Regional Primate Research Center, 505 NW 185th Ave., Beaverton, OR 97006. FAX: 503 614 3725; schatten{at}ohsu.edu
3 Current address: Oregon Regional Primate Research Center, 505 NW 185th Ave., Beaverton, OR 97006.
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