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Interferon- Modulates Phorbol Ester-Induced Production of Prostaglandin and Expression of Cyclooxygenase-2 and Phospholipase-A₂ from Bovine Endometrial Cells¹

^a Department of Dairy and Poultry Sciences, University of Florida, Gainesville, Florida 32611 ^b Centre de Recherche en Reproduction Animale, Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, Quebec, Canada J2S 7C6 ^c Department of Animal Science, University of Wyoming, Laramie, Wyoming 82071

ABSTRACT

Antiluteolytic actions of bovine interferon-tau (bIFN-) require suppression of prostaglandin F₂ (PGF₂) production. Our objective was to test whether bIFN- could block PGF₂ production and synthesis of phospholipase A₂ (PLA₂) and cyclooxygenase-2 (COX-2) enzymes induced by a protein kinase C (PKC) stimulator (phorbol 12,13 dibutyrate; PDBu). Bovine endometrial epithelial (BEND) cells were treated with PDBu in the presence or absence of bIFN-. Medium samples were analyzed for concentrations of PGF₂, whole-cell extracts were analyzed for abundance of PLA₂ and COX-2 by immunoblotting, and RNA extracts were examined for steady-state levels of COX-2 mRNA by Northern blotting. The PDBu stimulated production of PGF₂ between 3 and 12 h, levels of COX-2 mRNA by 3 h and protein expression of COX-2 and PLA₂ by 6 and 12 h, respectively. Added concomitantly with PDBu, bIFN- suppressed PGF₂ production, steady-state levels of COX-2 mRNA, and expression of COX-2 and PLA₂ proteins. Added after a 3-h stimulation with PDBu alone, bIFN- suppressed PGF₂ production after 1 h. Bovine IFN- inhibited intracellular mechanisms responsible for PGF₂ production in BEND cells, and this could be through both cytosolic and nuclear actions.

FOOTNOTES

First decision: 27 January 2000.

¹ This work was supported partially by grant 98–35203-6367, NRI Competitive Grants Program/USDA, Florida Agricultural Experimental Station Journal Series R-07419, by NIH grant NIH HD 32475–06, and by MRC of Canada, grant MT-13190.

² Correspondence: William W. Thatcher, Dept. of Dairy and Poultry Sciences, University of Florida, Shealy Dr. and Ritchie Rd., Gainesville, FL 32611. FAX: 352 392 5595; thatcher{at}dps.ufl.edu

³ Current address: Dept. of Animal and Range Sciences, North Dakota State University, Fargo, ND 58105.

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