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Differential Expression of Inositol 1,4,5-Trisphosphate Receptor Types 1, 2, and 3 in Rat Myometrium and Endometrium During Gestation¹

^a INSERM U442, Signalisation Cellulaire et Calcium, ^b Signalisation et Régulations Cellulaires, CNRS, IFR-FR 46, Université Paris-Sud, F-91405 Orsay Cedex, France

ABSTRACT

The regulation of the phospholipase C (PLC) and the expression of inositol 1,4,5-trisphosphate receptors (IP₃Rs) in terms of mRNA, proteins, and binding capacity were examined in the rat myometrium and endometrium at midgestation (Day 12) and at term (Day 21) comparatively to the estrogen-treated tissues (Day 0). In both uterine tissues, the production of inositol phosphates mediated by carbachol as well as by AlF₄^- was enhanced with advancing gestation. ³[H]IP₃ binding sites in membranes also increased during pregnancy (Day 21 > Day 12 > Day 0). The mRNAs encoding for three isoforms of IP₃R as well as their corresponding proteins, IP₃R-1, IP₃R-2, and IP₃R-3 were coexpressed, albeit to different extents, in the myometrium and endometrium. The expression of IP₃Rs increased with advancing gestation, except for IP₃R-2 that increased only in the endometrium at term. Thus, the pregnancy-related upregulation of the PLC cascade coincided with an increase in the expression of IP₃Rs. The difference noted between the two uterine tissues suggests that IP₃Rs may have cell-specific functions.

FOOTNOTES

First decision: 11 February 2000.

¹ J.E.M. is the recipient of "Poste Vert from INSERM" and "Conseil Régional d'Ile-de-France." The first two authors contributed equally to the work presented here.

² Correspondence: Jean Pierre Mauger, INSERM U442, Signalisation Cellulaire et Calcium, Université Paris-Sud, Bâtiment 443, F-91405 Orsay Cedex, France. FAX: 33 1 69 15 58 93; jean-pierre.mauger{at}ibaic.u-psud.fr

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