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The Rat Endozepine-Like Peptide Gene Is Highly Expressed in Late Haploid Stages of Male Germ Cell Development¹

^a Institute of Anatomy, University Hospital, Hamburg-Eppendorf, 20246 Hamburg, Germany ^b Institute for Hormone and Fertility Research, University of Hamburg, 22529 Hamburg, Germany ^c Institute for Reproductive Medicine, University of Münster, 48129 Münster, Germany

ABSTRACT

The structure of the endozepine-like peptide (ELP) gene is closely related to the intracellular acyl-CoA binding protein (ACBP), but unlike the generalized distribution of the latter, it is restricted to the male germ cells of the testis. In the present study, a combination of nonradioactive in situ mRNA hybridization and immunohistochemistry was used to precisely determine the cellular expression patterns of ELP mRNA and protein in control and methoxyacetic acid (MAA)-treated rat testes. ELP transcripts are first detectable in late stages (step 6) of round spermatids, with transcription increasing through late-elongating steps. Translation of the ELP mRNA is delayed, with first immunohistochemical staining occurring in elongated spermatids at step 16, and protein accumulating through step 19. ELP immunoreactivity proves to be an excellent marker for late spermatid stages and highlights the presumably clonal recovery of spermatids following MAA treatment.

FOOTNOTES

First decision: 28 March 2000.

¹ This project has been supported by the Hamburg-Münster Confocal Research Group of the Deutsche Forschungsgemeinschaft (Iv7/4-2, Ni130/15-4).

² Correspondence: Richard Ivell, Institute for Hormone and Fertility Research, University of Hamburg, Grandweg 64, 22529 Hamburg, Germany. FAX: 49 40 56190864; ivell{at}ihf.de

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