| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular article |
a School of Bioresources, Hiroshima Prefectural University, Shobara, Hiroshima 727-0023, Japan
ABSTRACT
The present study was conducted to examine the protective effect of cumulus cells on oocyte damage caused by reactive oxygen species (ROS), generated by the hypoxanthine-xanthine oxidase (XOD) system, during in vitro maturation of porcine oocytes. Cumulus-oocyte complexes (COCs) and cumulus-denuded oocytes (DOs) were cultured for 44 h in NCSU37 supplemented with cysteine, gonadotropins, 10% porcine follicular fluid, and hypoxanthine in the presence or absence of XOD. DNA cleavage and damage were analyzed using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) method and single cell microgel electrophoresis (comet) assay, respectively, and caspase-3 activity and glutathione (GSH) content were measured in each experimental group. Exposure of DOs to ROS resulted in meiotic arrest and the increase of degenerated oocytes. These degenerated DOs underwent apoptosis, as shown by the TUNEL-positive reaction within their germinal vesicles and the activation of caspase-3. The length of DNA migration in DOs treated with XOD was significantly longer than that of untreated DOs (P < 0.05). However, irreparable cell damage caused by ROS was not observed in COCs, and no difference was observed in the caspase-3 activity of both COCs treated with and without XOD. A significantly (P < 0.05) high level of GSH was found in COCs after 44 h of culture, compared with that of oocytes freshly isolated from their follicles, whereas GSH content in DOs markedly decreased after treatment with or without XOD. These findings suggest that cumulus cells have a critical role in protecting oocytes against oxidative stress-induced apoptosis through the enhancement of GSH content in oocytes.
First decision: 22 March 2000.
1 This study was supported by a grant from the Ministry of Education, Science and Culture of Japan (no. 11760196).
2 Correspondence. FAX: 81 8247 4 0191; hidettmt{at}bio.hiroshima-pu.ac.jp
This article has been cited by other articles:
|
|
 |
|
  A. Bettegowda, O. V. Patel, K.-B. Lee, K.-E. Park, M. Salem, J. Yao, J. J. Ireland, and G. W. Smith Identification of Novel Bovine Cumulus Cell Molecular Markers Predictive of Oocyte Competence: Functional and Diagnostic Implications Biol Reprod, August 1, 2008; 79(2): 301 - 309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Assidi, I. Dufort, A. Ali, M. Hamel, O. Algriany, S. Dielemann, and M.-A. Sirard Identification of Potential Markers of Oocyte Competence Expressed in Bovine Cumulus Cells Matured with Follicle-Stimulating Hormone and/or Phorbol Myristate Acetate In Vitro Biol Reprod, August 1, 2008; 79(2): 209 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P.A. van Montfoort, J. P.M. Geraedts, J. C.M. Dumoulin, A. P.M. Stassen, J. L.H. Evers, and T. A.Y. Ayoubi Differential gene expression in cumulus cells as a prognostic indicator of embryo viability: a microarray analysis Mol. Hum. Reprod., March 1, 2008; 14(3): 157 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, X.-H. Li, X. Ma, Z.-H. Wang, S. Lu, and Y.-L. Guo Redox-Induced Apoptosis of Human Oocytes in Resting Follicles In Vitro Reproductive Sciences, September 1, 2006; 13(6): 451 - 458. [Abstract] [PDF]
|
|
|
|
|
|
C. LaRosa and S. M. Downs Stress Stimulates AMP-Activated Protein Kinase and Meiotic Resumption in Mouse Oocytes Biol Reprod, March 1, 2006; 74(3): 585 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Pocar, D. Nestler, M. Risch, and B. Fischer Apoptosis in bovine cumulus-oocyte complexes after exposure to polychlorinated biphenyl mixtures during in vitro maturation Reproduction, December 1, 2005; 130(6): 857 - 868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Georgiou, E. Sostaric, C. H. Wong, A. P. L. Snijders, P. C. Wright, H. D. Moore, and A. Fazeli Gametes Alter the Oviductal Secretory Proteome Mol. Cell. Proteomics, November 1, 2005; 4(11): 1785 - 1796. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R Gonzalez-Fernandez, F Gaytan, E Martinez-Galisteo, P Porras, C A Padilla, J E Sanchez Criado, and J A Barcena Expression of glutaredoxin (thioltransferase) in the rat ovary during the oestrous cycle and postnatal development J. Mol. Endocrinol., June 1, 2005; 34(3): 625 - 635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Blomberg, E. L. Long, T. S. Sonstegard, C. P. Van Tassell, J. R. Dobrinsky, and K. A. Zuelke Serial analysis of gene expression during elongation of the peri-implantation porcine trophectoderm (conceptus) Physiol Genomics, January 20, 2005; 20(2): 188 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Roth and P.J. Hansen Involvement of Apoptosis in Disruption of Developmental Competence of Bovine Oocytes by Heat Shock During Maturation Biol Reprod, December 1, 2004; 71(6): 1898 - 1906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tatemoto, N. Muto, I. Sunagawa, A. Shinjo, and T. Nakada Protection of Porcine Oocytes Against Cell Damage Caused by Oxidative Stress During In Vitro Maturation: Role of Superoxide Dismutase Activity in Porcine Follicular Fluid Biol Reprod, October 1, 2004; 71(4): 1150 - 1157. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Pocar, R. Augustin, and B. Fischer Constitutive Expression of CYP1A1 in Bovine Cumulus Oocyte-Complexes in Vitro: Mechanisms and Biological Implications Endocrinology, April 1, 2004; 145(4): 1594 - 1601. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tatemoto, K. Ootaki, K. Shigeta, and N. Muto Enhancement of Developmental Competence after In Vitro Fertilization of Porcine Oocytes by Treatment with Ascorbic Acid 2-O-{alpha}-Glucoside During In Vitro Maturation Biol Reprod, December 1, 2001; 65(6): 1800 - 1806. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
