| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular Article |
a Department of Medicine, Flinders University of South Australia, Bedford Park, South Australia 5042, Australia
b Department of Chemical Pathology, Women's and Children's Hospital, North Adelaide, South Australia 5006, Australia
ABSTRACT
Proteoglycans (PGs) consist of a core protein and attached glycosaminoglycans (GAGs) and have diverse roles in cell and tissue biology. In follicles PGs have been detected only in follicular fluid and in cultured granulosa cells, and the composition of their GAGs has been determined. To identify PGs in whole ovarian follicles, not just in follicular fluid and granulosa cells, small (1–3-mm) bovine follicles were harvested. A proportion of these was incubated with 35SO4 for 24 h to incorporate radiolabel into the GAGs. The freshly harvested and cultured follicles were sequentially extracted with 6 M urea buffer, the same buffer with 0.1% Triton X-100 and then with 0.1 M NaOH. Proteoglycans were subjected to ion-exchange and size-exclusion chromatography. The GAGs were analyzed by chemical and enzymic digestion, and on the basis of their composition, we chose a list of known PGs to measure by ELISA analyses. Versican, perlecan, decorin, but not aggrecan or biglycan, were identified. These, excluding decorin for technical reasons, as well as a basal lamina glycoprotein, nidogen/entactin, were immunolocalized. Versican was localized to the thecal layers, including externa and the interna particularly in an area adjacent to the follicular basal lamina. Perlecan and nidogen were localized to the follicular basal lamina of antral follicles, both healthy and atretic, but not to that of preantral follicles. Both were localized to subendothelial basal laminas, but the former was not readily detected in arteriole smooth muscle layers. This study has confirmed the presence of versican and perlecan, but not the latter as a component of follicular fluid, and identified decorin and nidogen in ovarian antral follicles.
1 This work was supported by grants from the National Health and Medical Research Council of Australia, The Flinders Medical Centre Foundation, and The Flinders University of South Australia.
2 Correspondence. FAX: 61 8 8204 5450; ray.rodgers{at}flinders.edu.au
This article has been cited by other articles:
|
|
 |
|
  A. I. de Agostini, J.-C. Dong, C. de Vantery Arrighi, M.-A. Ramus, I. Dentand-Quadri, S. Thalmann, P. Ventura, V. Ibecheole, F. Monge, A.-M. Fischer, et al. Human Follicular Fluid Heparan Sulfate Contains Abundant 3-O-Sulfated Chains with Anticoagulant Activity J. Biol. Chem., October 17, 2008; 283(42): 28115 - 28124. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Wahlberg, A. Nylander, N. Ahlskog, K. Liu, and T. Ny Expression and Localization of the Serine Proteases High-Temperature Requirement Factor A1, Serine Protease 23, and Serine Protease 35 in the Mouse Ovary Endocrinology, October 1, 2008; 149(10): 5070 - 5077. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. K. Woodruff and L. D. Shea The Role of the Extracellular Matrix in Ovarian Follicle Development Reproductive Sciences, December 1, 2007; 14(8_suppl): 6 - 10. [Abstract] [PDF]
|
|
|
|
 |
|
 H. F. Irving-Rodgers, B. E. Friden, S. E. Morris, H. D. Mason, M. Brannstrom, K. Sekiguchi, N. Sanzen, L. M. Sorokin, Y. Sado, Y. Ninomiya, et al. Extracellular matrix of the human cyclic corpus luteum Mol. Hum. Reprod., September 1, 2006; 12(9): 525 - 534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. G Clarke, S. A Hope, S. Byers, and R. J Rodgers Formation of ovarian follicular fluid may be due to the osmotic potential of large glycosaminoglycans and proteoglycans Reproduction, July 1, 2006; 132(1): 119 - 131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ndiaye, T. Fayad, D. W. Silversides, J. Sirois, and J. G. Lussier Identification of Downregulated Messenger RNAs in Bovine Granulosa Cells of Dominant Follicles Following Stimulation with Human Chorionic Gonadotropin Biol Reprod, August 1, 2005; 73(2): 324 - 333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Richards, I. Hernandez-Gonzalez, I. Gonzalez-Robayna, E. Teuling, Y. Lo, D. Boerboom, A. E. Falender, K. H. Doyle, R. G. LeBaron, V. Thompson, et al. Regulated Expression of ADAMTS Family Members in Follicles and Cumulus Oocyte Complexes: Evidence for Specific and Redundant Patterns During Ovulation Biol Reprod, May 1, 2005; 72(5): 1241 - 1255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Melrose, S. Smith, and J. Whitelock Perlecan Immunolocalizes to Perichondrial Vessels and Canals in Human Fetal Cartilaginous Primordia in Early Vascular and Matrix Remodeling Events Associated with Diarthrodial Joint Development J. Histochem. Cytochem., November 1, 2004; 52(11): 1405 - 1413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Mittaz, D.L. Russell, T. Wilson, M. Brasted, J. Tkalcevic, L.A. Salamonsen, P.J. Hertzog, and M.A. Pritchard Adamts-1 Is Essential for the Development and Function of the Urogenital System Biol Reprod, April 1, 2004; 70(4): 1096 - 1105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Fayad, V. Levesque, J. Sirois, D. W. Silversides, and J. G. Lussier Gene Expression Profiling of Differentially Expressed Genes in Granulosa Cells of Bovine Dominant Follicles Using Suppression Subtractive Hybridization Biol Reprod, February 1, 2004; 70(2): 523 - 533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Madan, P. J. Bridges, C. M. Komar, A. G. Beristain, R. Rajamahendran, J. E. Fortune, and C. D. MacCalman Expression of Messenger RNA for ADAMTS Subtypes Changes in the Periovulatory Follicle after the Gonadotropin Surge and During Luteal Development and Regression in Cattle Biol Reprod, November 1, 2003; 69(5): 1506 - 1514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Melrose, S. Smith, P. Ghosh, and J. Whitelock Perlecan, the Multidomain Heparan Sulfate Proteoglycan of Basement Membranes, Is also a Prominent Component of the Cartilaginous Primordia in the Developing Human Fetal Spine J. Histochem. Cytochem., October 1, 2003; 51(10): 1331 - 1341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Russell, S. A. Ochsner, M. Hsieh, S. Mulders, and J. S. Richards Hormone-Regulated Expression and Localization of Versican in the Rodent Ovary Endocrinology, March 1, 2003; 144(3): 1020 - 1031. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Richards, D. L. Russell, S. Ochsner, M. Hsieh, K. H. Doyle, A. E. Falender, Y. K. Lo, and S. C. Sharma Novel Signaling Pathways That Control Ovarian Follicular Development, Ovulation, and Luteinization Recent Prog. Horm. Res., January 1, 2002; 57(1): 195 - 220. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
