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ARTICLES |
-Induced Oxytocin Secretion and Intracellular Calcium Mobilization in Cultured Bovine Luteal Cells1
a Laboratory of Reproductive Endocrinology, Faculty of Agriculture, Okayama University, Okayama 700-8530, Japan
b Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-718 Olsztyn-Kortowo, Poland
Although prostaglandin (PG) F2
released from the uterus has been shown to cause regression of the bovine corpus luteum (CL), the neuroendocrine, paracrine, and autocrine mechanisms regulating luteolysis and PGF2 action in the CL are not fully understood. A number of substances produced locally in the CL may be involved in maintaining the equilibrium between luteal development and its regression. The present study was carried out to determine whether noradrenaline (NA) and nitric oxide (NO) regulate the sensitivity of the bovine CL to PGF2 in vitro and modulate a positive feedback cascade between PGF2 and luteal oxytocin (OT) in cows. Bovine luteal cells (Days 8–12 of the estrous cycle) cultured in glass tubes were pre-exposed to NA (10-5 M) or an NO donor (S-nitroso-N-acetylpenicillamine [S-NAP]; 10-4 M) before stimulation with PGF2 (10-6 M). Noradrenaline significantly stimulated the release of progesterone (P4), OT, PGF2, and PGE2 (P < 0.01); however, S-NAP inhibited P4 and OT secretion (P < 0.05). Oxytocin secretion and the intracellular level of free Ca2+ ([Ca2+]i) were measured as indicators of CL sensitivity to PGF2. Prostaglandin F2 increased both the amount of OT secretion and [Ca2+]i by approximately two times the amount before (both P < 0.05). The S-NAP amplified the effect of PGF2 on [Ca2+]i and OT secretion (both P < 0.001), whereas NA diminished the stimulatory effects of PGF2 on [Ca2+]i (P < 0.05). Moreover, PGF2 did not exert any additionally effects on OT secretion in NA-pretreated cells. The overall results suggest that adrenergic and nitrergic agents play opposite roles in the regulation of bovine CL function. While NA stimulates P4 and OT secretion, NO may inhibit it in bovine CL. Both NA and NO are likely to stimulate the synthesis of luteal PGs and to modulate the action of PGF2. Noradrenaline may be the factor that is responsible for the limited action of PGF2 on CL and may be involved in the protection of the CL against premature luteolysis. In contrast, NO augments PGF2 action on CL and it may be involved in the course of luteolysis.
1 This research was supported by Grants-in-Aid for Scientific Research (nos. 11460129 and 11556054) from the Ministry of Education, Science, Sports and Culture of Japan, the Polish National Research Council (grant KBN 5 P06K 027 13), and by the Japanese Society for the Promotion of Science (JSPS). D.J.S. was a postdoctoral fellow supported by the JSPS (no. 96346).
2 Correspondence. FAX: 81 86 251 8388; kokuda{at}cc.okayama-u.ac.jp
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