| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
ARTICLES |
a The Jackson Laboratory, Bar Harbor, Maine 04609
The germ cell and somatic cell compartments of newborn rat and mouse ovaries, which contain only primordial stage follicles, were completely exchanged and reaggregated to produce xenogeneic chimeric ovaries. The reaggregated ovaries were grafted beneath the renal capsules of ovariectomized SCID mice to develop for periods up to 21 days. Xenogeneic follicles developed with essentially normal morphological characteristics. Both rat and mouse oocytes with species-specific characteristics grew within follicles that were composed of somatic cells exclusively of the alternative species. Rat oocytes grown in mouse follicles became competent to resume meiosis, and progressed to metaphase II when they were removed from follicles and cultured. In addition, mouse oocytes grown in rat follicles underwent fertilization and preimplantation development in vitro, and developed to term after embryos were transferred to pseudopregnant mouse foster mothers. Therefore, despite an estimated 11 million years of divergent evolution, oocytes and somatic cells of rat and mouse ovaries can be exchanged and can produce functional oocytes. It is concluded that factors involved in oocyte-somatic cell interactions necessary to support oocyte development and appropriate differentiation of the oocyte-associated granulosa cells are conserved between rats and mice. Moreover, although granulosa cells play important roles in oocyte development, the development of species-specific characteristics of oocytes occurs without apparent modification by a xenogeneic follicular environment.
1 This research was funded by the National Institute of Child Health and Human Development (NICHD) through NIH grant HD23839. The development of culture systems was supported by The National Cooperative Program on Non-Human In Vitro Fertilization and Preimplantation Development, which was funded by NICHD through cooperative agreement HD21970. The scientific services of the Jackson Laboratory receive support from a Cancer Center Core Grant (CA34196) from the National Cancer Institute.
2 Correspondence. FAX: 207 288 6073; jje{at}jax.org
This article has been cited by other articles:
|
|
 |
|
  G. FitzHarris, V. Siyanov, and J. M. Baltz Granulosa cells regulate oocyte intracellular pH against acidosis in preantral follicles by multiple mechanisms Development, December 1, 2007; 134(23): 4283 - 4295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. FitzHarris and J. M. Baltz Granulosa cells regulate intracellular pH of the murine growing oocyte via gap junctions: development of independent homeostasis during oocyte growth Development, February 15, 2006; 133(4): 591 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. I. Gittens and G. M. Kidder Differential contributions of connexin37 and connexin43 to oogenesis revealed in chimeric reaggregated mouse ovaries J. Cell Sci., November 1, 2005; 118(21): 5071 - 5078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. I. Gittens, K. J. Barr, B. C. Vanderhyden, and G. M. Kidder Interplay between paracrine signaling and gap junctional communication in ovarian follicles J. Cell Sci., January 1, 2005; 118(1): 113 - 122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Gosden Germline stem cells in the postnatal ovary: is the ovary more like a testis? Hum. Reprod. Update, May 1, 2004; 10(3): 193 - 195. [Full Text] [PDF]
|
|
|
|
 |
|
 D F Albertini Micromanagement of the ovarian follicle reserve - do stem cells play into the ledger? Reproduction, May 1, 2004; 127(5): 513 - 514. [Full Text] [PDF]
|
|
|
|
 |
|
 W. A. Roscoe, K. J. Barr, A. Amir Mhawi, D. K. Pomerantz, and G. M. Kidder Failure of Spermatogenesis in Mice Lacking Connexin43 Biol Reprod, September 1, 2001; 65(3): 829 - 838. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
