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Biology of Reproduction 63, 1098-1105 (2000)
© 2000 Society for the Study of Reproduction, Inc.

ARTICLES

Differential Expression of Estrogen Receptor-{alpha} and -ß and Androgen Receptor in the Ovaries of Marmosets and Humans

Philippa T.K. Saunders1,,a, Michael R. Millara, Karin Williamsa, Sheila Macphersona, Diana Harkissa, Richard A. Andersona, Brigid Orra, Nigel P. Groomeb, Graeme Scobiea, and Hamish M. Fraser

a MRC Human Reproductive Sciences Unit, Edinburgh, EH3 9ET, United Kingdom b Oxford Brookes University, Oxford, OX3 0BP, United Kingdom

Estrogens and androgens are essential for the maturation of the ovarian follicle and normal fertility in the female. We have used antibodies specific for both forms of estrogen receptor (alpha [ER{alpha}] and beta [ERß]) and androgen receptor (AR) to investigate the pattern of receptor expression in ovaries obtained from women and from a New World primate, the Common marmoset (Callthrix jacchus). On Western blots, three antibodies directed against different peptides within human ERß all recognized recombinant human (h) ERß but did not bind to recombinant hER{alpha}. The ERß protein was extracted from human ovary and prostate and marmoset ovary. In marmoset and human ovaries, ERß protein was detected in the nuclei of granulosa cells in all sizes of follicle (both before and after formation of the antrum), and it was also detected in thecal cells, corpora lutea, surface epithelium, and stroma. In contrast, ER{alpha} protein was not detected in the nuclei of granulosa cells in preantral follicles, was low/absent from stromal and thecal cells, but was expressed in granulosa cells of antral follicles and in the surface epithelium. The pattern of expression of AR protein more closely resembled that of ERß than ER{alpha}. In conclusion, three independent antibodies have demonstrated convincingly that ERß is expressed in a wide range of cells in the primate ovary. Granulosa cells in preantral follicles could contain ERß:ß dimers. In antral follicles, however, ER{alpha} is also expressed, and the formation of homo- or heterodimers containing ER{alpha} may influence the pattern of gene activation within these cells.

First decision: 17 February 2000.

1 Correspondence: P.T.K. Saunders, MRC Human Reproductive Sciences Unit, 37 Chalmers St., Edinburgh, EH3 9ET, UK. FAX: 44 131 228 5571; p.saunders{at}ed.ac.uk




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