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a Department of Clinical Studies, Reproduction,
b Department of Anatomy and Physiology,
c Department of Mathematics and Physics, Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Denmark
Availability of embryos of high quality is required to obtain satisfactory embryonic developmental rates and normal calves following transfer of in vitro-produced (IVP) bovine embryos. One relevant quality parameter is the frequency of chromosome aberrations, which can be evaluated using multicolor fluorescent in situ hybridization (FISH) with chromosome 6- and chromosome 7-specific probes in cattle. In this study, interphase nuclei (n = 3805) were analyzed from 426 bovine IVP embryos. We found that 73%, 72%, 81%, and 58% of the embryos from Days 2, 3, 4, and 5 post-insemination (pi), respectively, displayed a normal diploid chromosome number in all cells. When looking at the types of chromosome aberrations, the percentages of mixoploidy at Days 2, 3, 4, and 5 pi were 22%, 15%, 16%, and 42%, respectively, whereas the percentages of polyploidy (i.e., all nuclei in an embryo were analyzed and were polyploid) were 5%, 13%, 3%, and 0%, respectively. In conclusion, numerical chromosome aberrations were detected as early as Day 2 pi. The development of polyploid embryos is slow and is apparently arrested during the third cell cycle, whereas the mixoploid embryos seem to continue development.
1 Supported by the Danish Agricultural and Veterinary Research Council and Danish Biotechnology Program. Presented in part at the 32nd annual meeting of the Society for the Study of Reproduction, August 1999, Pullman, Washington.
2 Correspondence: Dorthe Viuff, Department of Clinical Studies, Reproduction, Royal Veterinary and Agricultural University, Dyrlægevej 68, DK-1870 Frederiksberg C, Denmark. FAX: 45 35 28 29 72; dv{at}kvl.dk
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