HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lysiak, J. J. Articles by Turner, T. T. Search for Related Content PubMed PubMed Citation Articles by Lysiak, J. J. Articles by Turner, T. T. Agricola Articles by Lysiak, J. J. Articles by Turner, T. T.

Fluctuations in Rat Testicular Interstitial Oxygen Tensions Are Linked to Testicular Vasomotion: Persistence after Repair of Torsion¹

^a Departments of Urology and Cell Biology, ^b University of Virginia Health Science Center, Charlottesville, Virginia 22908

ABSTRACT

Testicular microvascular blood flow is known to exhibit vasomotion, which has been shown to be significantly altered in the short term following the repair of testicular torsion. This loss of vasomotion may ultimately be responsible for the loss of spermatogenesis observed after testicular torsion in rats. In the present study, testicular vasomotion and interstitial oxygen tensions were simultaneously measured prior to, during, and at various time points after repair of testicular torsion in the rat. Testicular torsion was induced by a 720° rotation of the testis for 1 h. Laser-Doppler flowmetry and an oxygen electrode were used to simultaneously measure vasomotion and interstitial oxygen tensions (PO₂), respectively. Pretorsion control testes had a mean blood flow of 16.3 ± 1.3 perfusion units (PU) and displayed vasomotion with a cycle frequency of 12 ± 0.2 cycles per minute and a mean amplitude of 4.2 ± 0.3 PU. Mean testicular interstitial PO₂ was 12.5 ± 2.6 mm Hg, which displayed a cyclical variation of 11.9 ± 0.4 cycles per minute with a mean amplitude of 2.8 ± 0.8 mm Hg. During the torsion period, both mean blood flow and interstitial PO₂ decreased to approximately zero. Upon detorsion, mean microvascular blood flow and mean interstitial PO₂ values returned to values that were not significantly different from pretorsion values within 30 min; however, vasomotion and PO₂ cycling did not return, even after 24 h. It was 7 days after the repair of torsion before a regular pattern of vasomotion and PO₂ cycling returned. These results demonstrate for the first time a correlation between testicular vasomotion and interstitial PO₂ cycling, and this correlation persists after the repair of testicular torsion.

FOOTNOTES

First decision: 24 May 2000.

¹ Supported by National Institutes of Health grant DK-53072.

² Correspondence: Jeffrey J. Lysiak, Department of Urology, Box 800422, University of Virginia Health System, Charlottesville, VA 22908. FAX: 804 924 8311; jl6n{at}virginia.edu

This article has been cited by other articles:

				J. J. Lysiak, J. L. Kirby, J. J. Tremblay, R. I. Woodson, M. A. Reardon, L. A. Palmer, and T. T. Turner Hypoxia-Inducible Factor-1{alpha} Is Constitutively Expressed in Murine Leydig Cells and Regulates 3{beta}-Hydroxysteroid Dehydrogenase Type 1 Promoter Activity J Androl, March 1, 2009; 30(2): 146 - 156. [Abstract] [Full Text] [PDF]

				T. T. Turner and J. J. Lysiak Oxidative Stress: A Common Factor in Testicular Dysfunction J Androl, September 1, 2008; 29(5): 488 - 498. [Abstract] [Full Text] [PDF]

				J. J. Lysiak, H. J. Bang, Q. A. T. Nguyen, and T. T. Turner Activation of the Nuclear Factor Kappa B Pathway Following Ischemia-Reperfusion of the Murine Testis J Androl, January 1, 2005; 26(1): 129 - 135. [Abstract] [Full Text] [PDF]

				D. J. Selvage, S. Y. Lee, L. H. Parsons, D. O. Seo, and C. L. Rivier A Hypothalamic-Testicular Neural Pathway Is Influenced by Brain Catecholamines, But Not Testicular Blood Flow Endocrinology, April 1, 2004; 145(4): 1750 - 1759. [Abstract] [Full Text] [PDF]

				J. J. Lysiak, Q. A. T. Nguyen, J. L. Kirby, and T. T. Turner Ischemia-Reperfusion of the Murine Testis Stimulates the Expression of Proinflammatory Cytokines and Activation of c-jun N-Terminal Kinase in a Pathway to E-Selectin Expression Biol Reprod, July 1, 2003; 69(1): 202 - 210. [Abstract] [Full Text] [PDF]

				J. D. Powell, R. Elshtein, D. J. Forest, and M. A. Palladino Stimulation of Hypoxia-Inducible Factor-1 Alpha (HIF-1{alpha}) Protein in the Adult Rat Testis Following Ischemic Injury Occurs Without an Increase in HIF-1{alpha} Messenger RNA Expression Biol Reprod, September 1, 2002; 67(3): 995 - 1002. [Abstract] [Full Text] [PDF]

				J. J. Lysiak, Q. A. T. Nguyen, and T. T. Turner Peptide and Nonpeptide Reactive Oxygen Scavengers Provide Partial Rescue of the Testis After Torsion J Androl, May 1, 2002; 23(3): 400 - 409. [Abstract] [Full Text] [PDF]

				J. J. Lysiak, S. D. Turner, Q. A. T. Nguyen, K. Singbartl, K. Ley, and T. T. Turner Essential Role of Neutrophils in Germ Cell-Specific Apoptosis Following Ischemia/Reperfusion Injury of the Mouse Testis Biol Reprod, September 1, 2001; 65(3): 718 - 725. [Abstract] [Full Text] [PDF]