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Regular Article |
Induces Distinct Physiological Responses in Porcine Corpora Lutea after Acquisition of Luteolytic Capacity1
a Endocrinology-Reproductive Physiology Program,
b Department of Dairy Science, and
c Department of Animal Science, University of Wisconsin-Madison, Madison, Wisconsin 53706
ABSTRACT
This study examines differences in intracellular responses to cloprostenol, a prostaglandin (PG)F2
analog, in porcine corpora lutea (CL) before (Day 9 of estrous cycle) and after (Day 17 of pseudopregnancy) acquisition of luteolytic capacity. Pigs on Day 9 or Day 17 were treated with saline or 500 µg cloprostenol, and CL were collected 10 h (experiment I) or 0.5 h (experiment III) after treatment. Some CL were cut into small pieces and cultured to measure progesterone and PGF2 secretion. In experiment I, progesterone remained high and PGF2 low in luteal incubations from either Day 9 or Day 17 saline-treated pigs. Cloprostenol increased PGF2 production 465% and decreased progesterone production 87% only from Day 17 luteal tissue. Cloprostenol induced prostaglandin G/H synthase (PGHS)-2 mRNA (0.5 h) and protein (10 h) in both groups. In cell culture, cloprostenol or phorbol 12,13-didecanoate (PDD) (protein kinase C activator), induced PGHS-2 mRNA in luteal cells from both groups. However, acute cloprostenol treatment (10 min) decreased progesterone production and increased PGF2 production only from Day 17 luteal cells. Thus, PGF2 production is induced by cloprostenol in porcine CL with luteolytic capacity (Day 17) but not in CL without luteolytic capacity (Day 9). However, this change in PGF2 production is not explained by a difference in induction of PGHS-2 mRNA or protein.
First decision: 18 April 2000.
1 This work was supported by NIH grant HD-32623.
2 Correspondence: Milo C. Wiltbank, Department of Dairy Science, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, WI 53706. FAX: 608 263 9412; wiltbank{at}calshp.cals.wisc.edu
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