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Regular Article |
a Department of Biology, Trinity University, San Antonio, Texas 78212
b Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030
c Department of Obstetrics and Gynecology, Kumamoto University School of Medicine, Kumamoto, Japan
ABSTRACT
The ovulatory process in mammals begins when an endogenous surge in LH circulates to the ovary and couples with receptors in the plasma membranes of granulosa cells in mature ovarian follicles. This study provides evidence that the ovulatory stimulus includes induction of the gene for regulator of G-protein signaling protein-2 (RGS2). Immature Wistar rats were primed with 10 IU eCG s.c., and 48 h later the 12-h ovulatory process was initiated by 10 IU hCG (a homolog of LH) s.c. Ovarian RNA was extracted at 0, 2, 4, 8, 12, and 24 h after injecting the animals with hCG. The RNA extracts were used for reverse transcription-polymerase chain reaction differential display to detect gene expression in the stimulated ovarian tissue. Two of the amplified cDNAs that were upregulated within 2 h after the ovaries had been stimulated by hCG were homologous to segments of the mouse gene for RGS2. In situ hybridization indicated that the RGS2 mRNA was expressed in the granulosa layer of mature follicles. In conclusion, the gene for RGS2, which is known to regulate membrane signaling pathways, is transcribed in ovarian follicles in response to an ovulatory dose of gonadotropin.
1 This work was supported by NSF grant 9870793 (L.L.E.), by a grant to support T.U. as a Research Fellow of The Lalor Foundation, Providence, Rhode Island (L.L.E.), and by NIH grant HD-16229 (J.S.R.).
2 Correspondence. FAX: 210 999 7229; lespey{at}trinity.edu
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