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Regular Article |
a Institute of Biological Chemistry, Academia Sinica, and
b Institute of Biochemical Sciences, College of Science, National Taiwan University, Taipei 106, Taiwan
ABSTRACT
We studied the effect of a mouse seminal vesicle autoantigen (SVA) on BSA-stimulated functions of mouse sperm. Uncapacitated, capacitated, and acrosome-reacted stages of sperm were morphologically scored, and the cellular zinc content was examined cytologically in a modified Tyrode solution at 37°C for 80 min. More than 85% of control cells remained uncapacitated. Addition of 0.3% SVA to the cell incubation did not affect the cell status. Approximately 65% of cells were capacitated in the incubation medium containing 0.3% BSA. Only 30% of the cells became capacitated after incubation with 0.3% BSA and 0.3% SVA together. The decapacitation effect by 0.3% SVA could be subdued by more than 3% BSA in the cell incubation. Whereas BSA did, SVA did not cause removal of Zn2+ from sperm, but SVA could suppress the BSA effect. The tyrosine phosphorylated proteins in sperm were detected after incubation in a modified HEPES medium containing 0.3% BSA and/or 0.3% SVA at 37°C for 90 min. Whereas BSA enhanced greatly, SVA did not cause phosphorylation of proteins in the range of Mr 40 000–120 000. The BSA-stimulated protein tyrosine phosphorylation could be suppressed by SVA in the cell incubation.
1 Supported in part by three grants (NSC 89-2311-B-001-016, 89-2311-B-002-038, and 89-2311-B-001-064) from National Science Council, Taiwan.
2 Correspondence: Yee-Hsiung Chen, Institute of Biochemical Sciences, College of Science, National Taiwan University, P.O. Box 23-106, Taipei 106, Taiwan. FAX: 886 2 23635038; bc304{at}gate.sinica.edu.tw
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