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Acquisition of Meiotic Competence in Mouse Oocytes: Absolute Amounts of p34^cdc2, Cyclin B1, cdc25C, and wee1 in Meiotically Incompetent and Competent Oocytes¹

^a Center for Research on Reproduction and Women's Health and ^b Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104

ABSTRACT

M-Phase promoting factor (MPF) is a complex of p34^cdc2 and cyclin B. Results of previous studies in which relative mass amounts of these cell cycle regulators were determined suggested that the accumulation of p34^cdc2, rather than cyclin B, could be a limiting factor in the acquisition of meiotic competence in mouse oocytes. Nevertheless, in the absence of measurements of the absolute amount of these components of MPF, it is possible that the molar amount of p34^cdc2 is in excess to that of cyclin B, i.e., the accumulation of p34^cdc2 is not a limiting factor. We report measurements of the absolute mass of p34^cdc2 and cyclin B1, as well as the two proximal regulators of MPF, namely cdc25C and wee1, in meiotically incompetent and competent mouse oocytes. We find that the numbers of molecules of p34^cdc2, cyclin B1, cdc25C, and wee1 in meiotically incompetent oocytes are 1.4 x 10⁶, 11.3 x 10⁶, 24.6 x 10⁶, 15.6 x 10⁶, respectively, and in meiotically competent oocytes the numbers are 14.3 x 10⁶, 95.5 x 10⁶, 80.0 x 10⁶, 40.1 x 10⁶, respectively. Thus, the concentration of cyclin B1 is always in excess to that of p34^cdc2, and this is consistent with the hypothesis that the accumulation of p34^cdc2 plays a role in the acquisition of meiotic competence. Last, the concentration of cdc25C is greater than that of wee1 and the concentration of each is greater than that of p34^cdc2 in both meiotically incompetent and competent oocytes.

FOOTNOTES

First decision: 5 June 2000.

¹ This research was supported by a grant from the NIH to G.S.K. and R.M.S. (HD 22732).

² Correspondence: Gregory S. Kopf, Rm. 1315, Biomedical Research Building II, Center for Research on Reproduction and Women's Health, University of Pennsylvania, 421 Curie Blvd., Philadelphia, PA 19104-6142. FAX: 215 573 4337; kopf{at}mail.med.upenn.edu

³ Current address: Department of Medical Chemistry, Kyoto University Faculty of Medicine, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.

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