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Biology of Reproduction 63, 1713-1720 (2000)
© 2000 Society for the Study of Reproduction, Inc.

Regular Article

Expression of Tenascin-C in Stromal Cells of the Murine Uterus During Early Pregnancy: Induction by Interleukin-1{alpha}, Prostaglandin E2, and Prostaglandin F2{alpha}

Naomi Nodaa, Hiroyuki Minouraa, Rika Nishiuraa, Nagayasu Toyodaa, Kyoko Imanaka-Yoshidab, Teruyo Sakakurab, and Toshimichi Yoshida1,,b

a Departments of Obstetrics and Gynecology and b Pathology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan

ABSTRACT

Tenascin-C (TN-C), an extracellular matrix glycoprotein, is known to be expressed in uterine stroma in the peri-implantation period. Examination of the spatiotemporal pattern during early pregnancy using immunohistochemistry and in situ hybridization revealed TN-C expression in the stroma beneath the luminal epithelia of the murine endometrium on Days 0 and 1 of pregnancy, subsequent disappearance, and reappearance on Day 4. After decidualization, tissue around the deciduoma was positive. In situ hybridization demonstrated TN-C production by the stromal cells adjacent to the epithelia. To investigate the regulation of TN-C expression in vitro, murine uterine stromal and epithelial cells were isolated and cultured. Addition of interleukin-1{alpha} (IL-1{alpha}) and prostaglandin E2 (PGE2) and F2{alpha} (PGF2{alpha}) induced TN-C expression in the stromal cells at both protein and mRNA levels, while the sex steroid hormones, progesterone and ß-estradiol, exerted little effect. Immunohistochemistry using anti-IL-1{alpha} antibody showed epithelial cells to be positive on Days 2–4 of pregnancy, and addition of progesterone but not ß-estradiol enhanced IL-1{alpha} expression in epithelial cells in vitro. In a culture insert system, TN-C expression by stromal cells cocultured with epithelial cells was induced by addition of progesterone alone that was blocked by additions of anti-IL-1{alpha} antibody. Collectively, these findings indicate that TN-C expression in the preimplantation period is under the control of progesterone, but not directly, possibly by the paracrine and autocrine intervention of IL-1{alpha} secreted by epithelial cells and PGE2 and PGF2{alpha} secreted by stromal cells.

FOOTNOTES

First decision: 30 March 2000.

1 Correspondence: Toshimichi Yoshida, Department of Pathology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan. FAX: 81 59 231 5210; t-yosida{at}doc.medic.mie-u.ac.jp




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