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Biology of Reproduction 63, 1735-1746 (2000)
© 2000 Society for the Study of Reproduction, Inc.

Regular Article

Changes in the Temporal and Spatial Expression of Hß58 During Formation and Maturation of the Chorioallantoic Placenta in the Rat1

Virginia Rider2,a, Stephanie R. Jonesa, Raymond T. Fostera, and Kazuhiko Imakawab

a Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri–Kansas City, Kansas City, Missouri 64110 b Laboratory of Animal Breeding, Faculty of Agriculture, University of Tokyo, Tokyo 113, Japan

ABSTRACT

Cloning and sequencing of a cDNA amplified by RNA fingerprinting at the implantation site of pregnant rats revealed 80% similarity with Hß58, previously shown to be essential for formation of the chorioallantoic placenta in the mouse. Hß58 mRNA was detected in the endometrium of hormonally sensitized rats stimulated to undergo decidualization and in the contralateral uterine horns lacking a decidual stimulus, indicating that uterine expression of Hß58 mRNA did not require decidualization or the presence of a blastocyst. Immunodetection in the early postimplantation uterus (Days 6–8 of pregnancy) showed Hß58 localized in the luminal and glandular epithelia and some stromal cells. Decidual cells at Day 6 of pregnancy expressed Hß58, and by Day 9 of pregnancy, the protein localized throughout the maternal decidua. The temporal and spatial distribution of Hß58 in the developing chorioallantoic placenta was assessed at Days 10, 12, and 14 of pregnancy. Immunoreactive Hß58 localized to erythroid cells within the developing fetal vasculature of the chorioallantoic primordia at Day 10 of pregnancy. By Day 12, the fetal vasculature extended into the placental labyrinth, and the erythroid stem cells continued to strongly express Hß58. At Day 14 of pregnancy, immunoreactivity became evident in the trophoblast giant cells and syncytiotrophoblast of the fetal placenta. As the chorioallantoic placenta matured (Day 18), Hß58 mRNA was 3.6-fold higher in the labyrinth compared with the junctional region. Stable cell lines (HRP/LRP) isolated from the rat labyrinthine placenta expressed Hß58 mRNA and protein. The expression pattern of Hß58 in maternal and fetal placental tissues and its early expression in fetal erythroid stem cells during formation and maturation of the chorioallantoic placenta suggest that Hß58 plays key roles in the regulatory networks that control hematopoietic development and placentation.

FOOTNOTES

First decision: 3 May 2000.

1 Supported in part by grants from the University of Missouri Research Board and the Sarah Morrison Fund.

2 Correspondence: Virginia Rider, Department of Biology, Pittsburg State University, 1701 South Broadway, Pittsburg, KS 66762. FAX: 316 235 4194; vrider{at}pittstate.edu






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