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Regular Article |
a The Jackson Laboratory, Bar Harbor, Maine 04609
ABSTRACT
Sperm from C57BL/6J, DBA/2J, BALB/cJ, 129S3/SvImJ, and FVB/NJ inbred mice were cryopreserved in 3% skim milk/18% raffinose cryoprotectant solution. The post-thaw sperm from all strains were evaluated for their viability and fertility by comparing them against B6D2F1 sperm used as a control. The protocol used for freezing mouse sperm was effective in different strains, because the motility was decreased by 50% after cryopreservation similar to other mammalian sperm. However, the progressive motility and the fertility of each inbred strain were affected differently. The C57BL/6J, BALB/cJ, and 129S3/SvImJ strains were the most affected; their fertility (two-cell cleavage) decreased from 70%, 34%, and 84% when using freshly collected sperm to 6%, 12%, and 6% when using frozen/thawed sperm, respectively. Live newborns derived from frozen/thawed sperm were obtained from all strains in the study. These results corroborate the genetic variation among strains with regard to fertility and susceptibility to cryopreservation.
1 Supported by National Institutes of Health grants RR09781 and RR01262 and as part of the NICHD/NCRR National Cooperative Program on Mouse Sperm Cryopreservation to L.E.M. through Cooperative Agreement RR15012.
2 Correspondence: Jorge M. Sztein, The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609. FAX: 207 288 6149; jms{at}jax.org
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