| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular article |
Has a Functional Role in the Mouse Rete Testis and Efferent Ductules1
a Department of Veterinary Bioscience, University of Illinois, Urbana, Illinois 61802
b Department of Animal Science, University of Illinois, Urbana, Illinois 61801
c Department of Biochemistry and
d Department of Veterinary Biomedical Sciences, University of Missouri, Columbia, Missouri 65211
ABSTRACT
Previous studies of the estrogen receptor-alpha knockout (
ERKO) in the male mouse demonstrate that the rete testis and efferent ductules are targets of estrogen. Because the ERKO mouse lacks a functional estrogen receptor alpha (ER) throughout development, it was not known whether the morphological and physiological abnormalities observed in the ERKO male were due to developmental defects or to dysfunctions concurrent with the lack of ER in the tissue. This study was designed to determine if treatment of normal wild-type (WT) mice with the pure antiestrogen, ICI 182,780, (ICI) could reproduce the morphological characteristics seen in ERKO mice. Thirty-day-old male mice were treated for 35 days with either castor oil or ICI. Age-equivalent ERKO mice were used for comparison. Light microscopic examinations of the reproductive tracts revealed dramatic changes in the efferent ductules of treated mice: a 1.7-fold increase in luminal diameter, a 56% reduction in epithelial cell height, a 60% reduction in brush boarder height of nonciliated cells, and an apparent reduction of the number of observable lysosomes and endocytotic vesicles. Testes of ICI-treated mice showed swollen rete testes area (6.5 times larger than control) and a 65% reduction in rete testis epithelium height. However, there were no significant changes in body and testis weights. These results indicate that ER blockage with ICI in WT mice results in morphological changes of the efferent ductules resembling those seen in ERKO siblings of the same age. Based on this study, we conclude that ER has a functional role in the mouse reproductive tract and the aberrant morphology observed in the efferent ductules of the ERKO mouse is likely the result of a concurrent response to the lack of functional ER, and not solely due to the lack of ER during early developmental times.
First decision: 11 April 2000.
1 This work was supported in part by NIH grant HD-35126. Part of this work was presented at the XV North American Testis Workshop, Louisville, Kentucky.
2 Correspondence: David Bunick, University of Illinois, Department of Veterinary Bioscience, 2001 South Lincoln Ave., Urbana, IL 61801. FAX: 217 244 1652; d-bunick{at}uiuc.edu
This article has been cited by other articles:
|
|
 |
|
  K L Hahn, B Beres, M. J Rowton, M K Skinner, Y Chang, A Rawls, and J Wilson-Rawls A deficiency of lunatic fringe is associated with cystic dilation of the rete testis Reproduction, January 1, 2009; 137(1): 79 - 93. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Yasuhara, G. R. O. Gomes, E. R. Siu, C. I. Suenaga, E. Marostica, C. S. Porto, and M. F. M. Lazari Effects of the Antiestrogen Fulvestrant (ICI 182,780) on Gene Expression of the Rat Efferent Ductules Biol Reprod, September 1, 2008; 79(3): 432 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A Pearl, E. At-Taras, T. Berger, and J. F Roser Reduced endogenous estrogen delays epididymal development but has no effect on efferent duct morphology in boars Reproduction, October 1, 2007; 134(4): 593 - 604. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Anway and M. K. Skinner Epigenetic Transgenerational Actions of Endocrine Disruptors Endocrinology, June 1, 2006; 147(6): s43 - s49. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E.E. At-Taras, A.J. Conley, T. Berger, and J.F. Roser Reducing Estrogen Synthesis Does Not Affect Gonadotropin Secretion in the Developing Boar Biol Reprod, January 1, 2006; 74(1): 58 - 66. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, C. A. Putnam-Lawson, H. Knapp-Hoch, P. J. Friel, D. Mitchell, R. Hively, and M. D. Griswold Immunolocalization and Regulation of Cystatin 12 in Mouse Testis and Epididymis Biol Reprod, November 1, 2005; 73(5): 872 - 880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A Oliveira, G. A B Mahecha, K. Carnes, G. S Prins, P. T K Saunders, L. R Franca, and R. A Hess Differential hormonal regulation of estrogen receptors ER{alpha} and ER{beta} and androgen receptor expression in rat efferent ductules Reproduction, July 1, 2004; 128(1): 73 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Aquila, D. Sisci, M. Gentile, E. Middea, S. Catalano, A. Carpino, V. Rago, and S. Ando Estrogen Receptor (ER){alpha} and ER{beta} Are Both Expressed in Human Ejaculated Spermatozoa: Evidence of Their Direct Interaction with Phosphatidylinositol-3-OH Kinase/Akt Pathway J. Clin. Endocrinol. Metab., March 1, 2004; 89(3): 1443 - 1451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Aquila, D. Sisci, M. Gentile, A. Carpino, E. Middea, S. Catalano, V. Rago, and S. Ando Towards a physiological role for cytochrome P450 aromatase in ejaculated human sperm Hum. Reprod., August 1, 2003; 18(8): 1650 - 1659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rivas, C. Mckinnell, J. S. Fisher, N. Atanassova, K. Williams, and R. M. Sharpe Neonatal Coadministration of Testosterone With Diethylstilbestrol Prevents Diethylstilbestrol Induction of Most Reproductive Tract Abnormalities in Male Rats J Androl, July 1, 2003; 24(4): 557 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Schlatt, A. Honaramooz, M. Boiani, H. R. Scholer, and I. Dobrinski Progeny from Sperm Obtained after Ectopic Grafting of Neonatal Mouse Testes Biol Reprod, June 1, 2003; 68(6): 2331 - 2335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.O. Goyal, A. Robateau, T.D. Braden, C.S. Williams, K.K. Srivastava, and K. Ali Neonatal Estrogen Exposure of Male Rats Alters Reproductive Functions at Adulthood Biol Reprod, June 1, 2003; 68(6): 2081 - 2091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rivas, J. S. Fisher, C. McKinnell, N. Atanassova, and R. M. Sharpe Induction of Reproductive Tract Developmental Abnormalities in the Male Rat by Lowering Androgen Production or Action in Combination with a Low Dose of Diethylstilbestrol: Evidence for Importance of the Androgen-Estrogen Balance Endocrinology, December 1, 2002; 143(12): 4797 - 4808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Pak, G. R. Lynch, and P.-S. Tsai Differential Alteration of the Reproductive Axis by Testosterone and Estrogen in Peripubertal and Adult Male Siberian Hamsters (Phodopus sungorus) Biol Reprod, September 1, 2002; 67(3): 706 - 711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Tong and W.-C. Song Estrogen Sulfotransferase: Discrete and Androgen-Dependent Expression in the Male Reproductive Tract and Demonstration of an in Vivo Function in the Mouse Epididymis Endocrinology, August 1, 2002; 143(8): 3144 - 3151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Aquila, D. Sisci, M. Gentile, E. Middea, L. Siciliano, and S. Ando Human Ejaculated Spermatozoa Contain Active P450 Aromatase J. Clin. Endocrinol. Metab., July 1, 2002; 87(7): 3385 - 3390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Oliveira, Q. Zhou, K. Carnes, R. Nie, D. E. Kuehl, G. L. Jackson, L. R. Franca, M. Nakai, and R. A. Hess ER Function in the Adult Male Rat: Short- and Long-Term Effects of the Antiestrogen ICI 182,780 on the Testis and Efferent Ductules, without Changes in Testosterone Endocrinology, June 1, 2002; 143(6): 2399 - 2409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Nie, Q. Zhou, E. Jassim, P. T.K. Saunders, and R. A. Hess Differential Expression of Estrogen Receptors {alpha} and {beta} in the Reproductive Tractsof Adult Male Dogs and Cats Biol Reprod, April 1, 2002; 66(4): 1161 - 1168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Qian, X. J. Sun, M. H. Tong, X. P. Li, J. Richa, and W.-C. Song Targeted Disruption of the Mouse Estrogen Sulfotransferase Gene Reveals a Role of Estrogen Metabolism in Intracrine and Paracrine Estrogen Regulation Endocrinology, December 1, 2001; 142(12): 5342 - 5350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-H. Lee, C. Finnigan-Bunick, J. Bahr, and D. Bunick Estrogen Regulation of Ion Transporter Messenger RNA Levels in Mouse Efferent Ductules Are Mediated Differentially Through Estrogen Receptor (ER) {alpha} and ER{beta} Biol Reprod, November 1, 2001; 65(5): 1534 - 1541. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Zhou, L. Clarke, R. Nie, K. Carnes, L.-W. Lai, Y.-H. H. Lien, A. Verkman, D. Lubahn, J. S. Fisher, B. S. Katzenellenbogen, et al. Estrogen action and male fertility: Roles of the sodium/hydrogen exchanger-3 and fluid reabsorption in reproductive tract function PNAS, October 31, 2001; (2001) 241245898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Telgmann, J.J. Brosens, K. Kappler-Hanno, R. Ivell, and C. Kirchhoff Epididymal epithelium immortalized by simian virus 40 large T antigen: a model to study epididymal gene expression Mol. Hum. Reprod., October 1, 2001; 7(10): 935 - 945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Oliveira, K. Carnes, L. R. Franca, and R. A. Hess Infertility and Testicular Atrophy in the Antiestrogen-Treated Adult Male Rat Biol Reprod, September 1, 2001; 65(3): 913 - 920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. O'Donnell, K. M. Robertson, M. E. Jones, and E. R. Simpson Estrogen and Spermatogenesis Endocr. Rev., June 1, 2001; 22(3): 289 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhou, L. Clarke, R. Nie, K. Carnes, L.-W. Lai, Y.-H. H. Lien, A. Verkman, D. Lubahn, J. S. Fisher, B. S. Katzenellenbogen, et al. From the Cover: Estrogen action and male fertility: Roles of the sodium/hydrogen exchanger-3 and fluid reabsorption in reproductive tract function PNAS, November 20, 2001; 98(24): 14132 - 14137. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
