| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular Article |
a Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada T2N 4N1
ABSTRACT
The objective of the present study was to identify and characterize transcripts whose levels are increased in the mouse uterus during decidualization. Using the method of suppression subtractive hybridization, we identified a novel transcript. This transcript contained a potential open reading frame that coded for a 196-amino-acid protein that shows homologies to the heat shock protein 20 family of genes. This transcript was expressed in several adult tissues and in the embryo. Its steady-state level was significantly greater in implantation segments of the uterus compared to nonimplantation segments. Furthermore, the steady-state levels of this novel transcript were significantly greater in uterine horns undergoing artificially induced decidualization compared to control contralateral horns. Using in situ hybridization methods, signals for the transcript were localized to the endometrial stromal cells that were undergoing decidualization. Finally, we found that progesterone caused a significant increase in the steady-state level of this novel transcript in the uterus when administered to ovariectomized mice. In the presence of estradiol-17ß, this effect was significantly reduced. In conclusion, we have identified a novel transcript of a potential heat shock protein whose level is significantly increased in the uterus during decidualization and in response to progesterone.
1 Supported by the Canadian Institutes of Health Research (G.A.S.) and the Lalor Foundation (B.B.). B.B. was the recipient of Lalor Foundation and the Alberta Heritage Foundation for Medical Research postdoctoral fellowships during this work.
2 Correspondence: Brent M. Bany, Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive N.W., Calgary, AB, Canada T2N 4N1. FAX: 403 270 0737; bany{at}ucalgary.ca
This article has been cited by other articles:
|
|
 |
|
  J. L. Herington, J. Bi, J. D. Martin, and B. M. Bany {beta}-Catenin (CTNNB1) in the Mouse Uterus During Decidualization and the Potential Role of Two Pathways in Regulating Its Degradation J. Histochem. Cytochem., September 1, 2007; 55(9): 963 - 974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B E Cross, H M O'Dea, and D J MacPhee Expression of small heat shock-related protein 20 (HSP20) in rat myometrium is markedly decreased during late pregnancy and labour Reproduction, April 1, 2007; 133(4): 807 - 817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B G White, S J Williams, K Highmore, and D J MacPhee Small heat shock protein 27 (Hsp27) expression is highly induced in rat myometrium during late pregnancy and labour Reproduction, January 1, 2005; 129(1): 115 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Dey, H. Lim, S. K. Das, J. Reese, B. C. Paria, T. Daikoku, and H. Wang Molecular Cues to Implantation Endocr. Rev., June 1, 2004; 25(3): 341 - 373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Austin, B. M. Bany, E. L. Belden, L. A. Rempel, J. C. Cross, and T. R. Hansen Interferon-Stimulated Gene-15 (Isg15) Expression Is Up-Regulated in the Mouse Uterus in Response to the Implanting Conceptus Endocrinology, July 1, 2003; 144(7): 3107 - 3113. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
