| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular Article |
b Department of Animal Science, Tokyo University of Agriculture, Kanagawa 243-0034, Japan
c Central Institute for Experimental Animals, Kawasaki-shi, Kanagawa 216-0001, Japan
ABSTRACT
Cloning using G0-arrested somatic cells has led to the suggestion that this stage of the cell cycle is necessary for the success of cloning. In this study we report that cloned mice can be generated from fetal fibroblasts arrested at metaphase of the cell cycle. The procedure involves fusing a metaphase-arrested fetal fibroblast to an enucleated oocyte. After parthenogenetic activation a polar body and single diploid pronucleus were formed. Some of these were allowed to develop to the blastocyst stage, while others were enucleated and the nucleus was transferred to an enucleated fertilized 1-cell embryo. After the single transfer technique, 2 out of 164 developed to late stages of gestation were dead with gross abnormalities. However, after the serial nuclear transfer, 5 out of 272 embryos were recovered live at Day 19.5, and 2 of these went on to develop into apparently normal adults. All of the cloned embryos showed severe placental hypertrophy and defective differentiation of placental tissues. This study illustrates that reprogramming can occur after nuclear transfer at metaphase of the cell cycle.
1 This work was supported by grants from the Ministry of Education, Science, Culture and Sports of Japan, the Ministry of Agriculture of Japan, and the Japanese Society for Promotion of Science.
2 Correspondence: Tomohiro Kono, Department of Animal Science, Tokyo University of Agriculture, 1737 Funako Atsugi-shi, Kanagawa 243-0034, Japan. FAX: 81 46 270 6575; tomohiro{at}nodai.ac.jp
This article has been cited by other articles:
|
|
 |
|
  Y. Jincho, Y. Sotomaru, M. Kawahara, Y. Ono, H. Ogawa, Y. Obata, and T. Kono Identification of Genes Aberrantly Expressed in Mouse Embryonic Stem Cell-Cloned Blastocysts Biol Reprod, April 1, 2008; 78(4): 568 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K Berg, C. Li, G. Asher, D. N Wells, and B. Oback Red Deer Cloned from Antler Stem Cells and Their Differentiated Progeny Biol Reprod, September 1, 2007; 77(3): 384 - 394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Misica-Turner, F. C. Oback, M. Eichenlaub, D. N. Wells, and B. Oback Aggregating Embryonic but Not Somatic Nuclear Transfer Embryos Increases Cloning Efficiency in Cattle Biol Reprod, February 1, 2007; 76(2): 268 - 278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Schurmann, D. N Wells, and B. Oback Early zygotes are suitable recipients for bovine somatic nuclear transfer and result in cloned offspring. Reproduction, December 1, 2006; 132(6): 839 - 848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Shimozawa, Y. Sotomaru, N. Eguchi, S. Suzuki, K. Hioki, T. Usui, T. Kono, and M. Ito Phenotypic abnormalities observed in aged cloned mice from embryonic stem cells after long-term maintenance. Reproduction, September 1, 2006; 132(3): 435 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R Arnold, V. Bordignon, R. Lefebvre, B. D Murphy, and L. C Smith Somatic cell nuclear transfer alters peri-implantation trophoblast differentiation in bovine embryos. Reproduction, August 1, 2006; 132(2): 279 - 290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ono and T. Kono Irreversible Barrier to the Reprogramming of Donor Cells in Cloning with Mouse Embryos and Embryonic Stem Cells Biol Reprod, August 1, 2006; 75(2): 210 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Skrzyszowska, Z. Smorag, R. Slomski, L. Katska-Ksiazkiewicz, R. Kalak, E. Michalak, K. Wielgus, J. Lehmann, D. Lipinski, M. Szalata, et al. Generation of Transgenic Rabbits by the Novel Technique of Chimeric Somatic Cell Cloning Biol Reprod, June 1, 2006; 74(6): 1114 - 1120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. V. Dindot, P. W. Farin, C. E. Farin, J. Romano, S. Walker, C. Long, and J. A. Piedrahita Epigenetic and Genomic Imprinting Analysis in Nuclear Transfer Derived Bos gaurus/Bos taurus Hybrid Fetuses Biol Reprod, August 1, 2004; 71(2): 470 - 478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-C. Ng, N. Chen, W.-Y. Yip, S.-L. Liow, G.-Q. Tong, B. Martelli, L. G. Tan, and P. Martelli The first cell cycle after transfer of somatic cell nuclei in a non-human primate Development, May 15, 2004; 131(10): 2475 - 2484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-C. Chang, Z. P. Nagy, R. Abdelmassih, X. Yang, and X. C. Tian Nuclear and Microtubule Dynamics of G2/M Somatic Nuclei During Haploidization in Germinal Vesicle-Stage Mouse Oocytes Biol Reprod, March 1, 2004; 70(3): 752 - 758. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J. Mullins, I. Wilmut, and J. J. Mullins Nuclear transfer in rodents J. Physiol., January 1, 2004; 554(1): 4 - 12. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Inoue, N. Ogonuki, K. Mochida, Y. Yamamoto, K. Takano, T. Kohda, F. Ishino, and A. Ogura Effects of Donor Cell Type and Genotype on the Efficiency of Mouse Somatic Cell Cloning Biol Reprod, October 1, 2003; 69(4): 1394 - 1400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Gomez, J. A. Jenkins, A. Giraldo, R. F. Harris, A. King, B. L. Dresser, and C. E. Pope Nuclear Transfer of Synchronized African Wild Cat Somatic Cells into Enucleated Domestic Cat Oocytes Biol Reprod, September 1, 2003; 69(3): 1032 - 1041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Bordignon, R. Keyston, A. Lazaris, A. S. Bilodeau, J. H.F. Pontes, D. Arnold, G. Fecteau, C. Keefer, and L. C. Smith Transgene Expression of Green Fluorescent Protein and Germ Line Transmission in Cloned Calves Derived from In Vitro-Transfected Somatic Cells Biol Reprod, June 1, 2003; 68(6): 2013 - 2023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Miyoshi, S. J. Rzucidlo, S. L. Pratt, and S. L. Stice Improvements in Cloning Efficiencies May Be Possible by Increasing Uniformity in Recipient Oocytes and Donor Cells Biol Reprod, April 1, 2003; 68(4): 1079 - 1086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Heindryckx, A. Rybouchkin, J. Van der Elst, and M. Dhont Serial Pronuclear Transfer Increases the Developmental Potential of In Vitro-Matured Oocytes in Mouse Cloning Biol Reprod, December 1, 2002; 67(6): 1790 - 1795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gao, B. Gasparrini, M. McGarry, T. Ferrier, J. Fletcher, L. Harkness, P. De Sousa, and I. Wilmut Germinal Vesicle Material Is Essential for Nucleus Remodeling after Nuclear Transfer Biol Reprod, September 1, 2002; 67(3): 928 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.H. Choi, C.C. Love, Y.G. Chung, D.D. Varner, M.E. Westhusin, R.C. Burghardt, and K. Hinrichs Production of Nuclear Transfer Horse Embryos by Piezo-Driven Injection of Somatic Cell Nuclei and Activation with Stallion Sperm Cytosolic Extract Biol Reprod, August 1, 2002; 67(2): 561 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Mitalipov, R. R. Yeoman, K. D. Nusser, and D. P. Wolf Rhesus Monkey Embryos Produced by Nuclear Transfer from Embryonic Blastomeres or Somatic Cells Biol Reprod, May 1, 2002; 66(5): 1367 - 1373. [Abstract] [Full Text]
|
|
|
|
|
|
Y. G. Chung, M. R.W. Mann, M. S. Bartolomei, and K. E. Latham Nuclear-Cytoplasmic "Tug of War" During Cloning: Effects of Somatic Cell Nuclei on Culture Medium Preferences of Preimplantation Cloned Mouse Embryos Biol Reprod, April 1, 2002; 66(4): 1178 - 1184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Humpherys, K. Eggan, H. Akutsu, K. Hochedlinger, W. M. Rideout III, D. Biniszkiewicz, R. Yanagimachi, and R. Jaenisch Epigenetic Instability in ES Cells and Cloned Mice Science, July 6, 2001; 293(5527): 95 - 97. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
