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Regular Article |
Stimulates the Janus Kinase-Signal Transducer and Activator of Transcription Pathway in Bovine Endometrial Epithelial Cells1
a Departments of Dairy and Poultry Sciences and
b Microbiology and Cell Science, University of Florida, Gainesville, Florida 32611
c Center for Animal Biotechnology and Genomics, Texas A&M University System Health Science Center, Texas A&M University, College Station, Texas 77843
d Department of Animal Science, University of Wyoming, Laramie, Wyoming 82071
ABSTRACT
Trophoblastic bovine interferon-tau (bIFN-
) suppresses luteolytic pulses of endometrial prostaglandin F2
(PGF2) at the time of maternal recognition of pregnancy. This results in maintenance of the corpus luteum in cattle. The hypothesis that effects of bIFN- in the endometrium were through activation of the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway of signal transduction was tested. Whole cell, cytosolic, and nuclear extracts from bovine endometrial cells treated with bIFN- were analyzed by immunoprecipitation, immunoblotting, and electrophoretic mobility shift assays in a series of dose- and time-dependency experiments. Bovine IFN- stimulated tyrosine phosphorylation, homo- and heterodimer formation, nuclear translocation, and DNA binding of STAT proteins 1, 2, and 3. Moreover, bIFN- induced synthesis of interferon-regulatory factor. In conclusion, bIFN- stimulates the JAK-STAT pathway in the bovine endometrium. It is proposed that activation of the JAK-STAT pathway is involved in regulating the antiluteolytic effects of bIFN-.
1 This work was supported partially by grant 98-35203-6367 from the NRI Competitive Grants Program/USDA, Florida Agricultural Experimental Station Journal Series no. R-07779, and by NIH grant NIH HD 32475-06.
2 Correspondence: William W. Thatcher, Dept. of Animal Sciences, Shealy Dr. and Ritchie Rd., Gainesville, FL 32611. FAX: 352 392 5595; thatcher{at}animal.ufl.edu
3 Current address: Centro de Biotecnologia em Reprodução Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Pirassununga, SP 13630-000, Brazil.
4 Current address: Institute de Reproducción Animal, Facultad de Ciencias Veterinarias, Universidad Central de Venezuela, Maracay, Aragua 2101, Venezuela.
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