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Actions of Prostaglandin F₂ and Prolactin on Intercellular Adhesion Molecule-1 Expression and Monocyte/Macrophage Accumulation in the Rat Corpus Luteum¹

^a Department of Animal and Nutritional Sciences, University of New Hampshire, Durham, New Hampshire 03824-3590 ^b Department of Animal Science, University of Wisconsin, Madison, Wisconsin 53706-1284

ABSTRACT

Expression of intercellular adhesion molecule-1 (ICAM-1) and the accumulation of monocytes/macrophages are inflammatory events that occur during PRL (PRL)-induced regression of the rat corpus luteum. Here we have compared the ability of prostaglandin F₂ (PGF) and PRL to induce, in rat corpora lutea, inflammatory events thought to perpetuate luteal regression. Immature rats were ovulated with eCG-hCG and then hypophysectomized (Day 0), which resulted in a single cohort of persistent, functional corpora lutea. On Days 9–11, the rats received twice daily injections of saline, PGF (Lutalyse, 250 µg/injection), or PRL (312 µg/injection) to induce luteal regression. Surprisingly, luteal weight and plasma progestin concentrations (progesterone and 20-dihydroprogesterone) did not differ between PGF-treated rats and controls; whereas both luteal weight and plasma progestins declined significantly in PRL-treated rats. Furthermore, corpora lutea of PGF-treated rats and controls contained relatively minimal ICAM-1 staining and few monocytes/macrophages. In contrast, but as expected, corpora lutea of PRL-treated rats stained intensely for ICAM-1 and contained numerous monocytes/macrophages. In an additional experiment, there was no indication that luteal prostaglandin F₂ receptor mRNA diminished as a result of hypophysectomy. These findings suggest that prolactin, not PGF, induces the inflammatory events that accompany regression of the rat corpus luteum.

FOOTNOTES

First decision: 25 August 2000.

¹ Supported by U.S. Department of Agriculture grant 98–35208-6654 and by funds from the University of New Hampshire Vice President for Research. This manuscript is scientific contribution number 2059 from the New Hampshire Agricultural Experiment Station.

² Correspondence: D.H. Townson, Department of Animal and Nutritional Sciences, Kendall Hall, 128 Main St., University of New Hampshire, Durham, NH 03824-3590. FAX: 603 862 3758; dave.townson{at}unh.edu

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