HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lee, K.-F. Articles by Yeung, W. S.B. Search for Related Content PubMed PubMed Citation Articles by Lee, K.-F. Articles by Yeung, W. S.B. Agricola Articles by Lee, K.-F. Articles by Yeung, W. S.B.

A Comparative Study of Gene Expression in Murine Embryos Developed In Vivo, Cultured In Vitro, and Cocultured with Human Oviductal Cells Using Messenger Ribonucleic Acid Differential Display¹

^a Department of Obstetrics and Gynaecology, Queen Mary Hospital, and ^b Department of Zoology, The University of Hong Kong, Hong Kong, China

ABSTRACT

The objectives of this study were to compare the mRNA expression patterns in early mouse embryos in different culture conditions by differential display reverse transcription-polymerase chain reaction (DDRT-PCR). Embryos developed in vivo, cultured in vitro, and cocultured with human oviductal epithelial cells were studied at the 2-cell, 4-cell, 8-cell/morula, and blastocyst stages. Messenger RNA profiles were displayed by DDRT-PCR using downstream T₁₁VV (V = A, C, or G) and upstream decamer primers. Total cDNA banding patterns were highly conserved in the three groups studied. Some fragments are unique in different culture conditions. Thirteen out of the 40 selected differentially expressed clones were characterized. The DNA sequence analyses of these clones displayed high sequence homology with cDNA sequences in the mouse expressed sequence tag database. Using semiquantitative RT-PCR, we confirmed differential expression of these DD amplicons in the three groups of embryos. The temporal expression of some of the selected DD amplicons during preimplantation development were studied in the three groups of embryos. In conclusion, DDRT-PCR is an effective tool for contrasting gene expression patterns and characterizing mRNA transcripts in mouse embryo.

FOOTNOTES

First decision: 26 September 2000.

¹ Supported by a grant from the Research Grant Council of the Hong Kong University Grant Committee (HKU7333/97M) to W.S.B.Y.

² Correspondence: Kai-Fai Lee, Department of Obstetrics and Gynaecology, Queen Mary Hospital, The University of Hong Kong, Pokfulam Rd., Hong Kong, China. FAX: 852 2855 0947; ckflee{at}hkucc.hku.hk

This article has been cited by other articles:

				K.-F. Lee, Y.-T. Tam, Y. Zuo, A. W.Y. Cheong, R. T.K. Pang, N. P.Y. Lee, C. K.Y. Shum, P.-C. Tam, A. N.Y. Cheung, Z.-M. Yang, et al. Characterization of an acrosome protein VAD1.2/AEP2 which is differentially expressed in spermatogenesis Mol. Hum. Reprod., August 1, 2008; 14(8): 465 - 474. [Abstract] [Full Text] [PDF]

				K.-F. Lee, J.-S. Xu, Y.-L. Lee, and W. S. B. Yeung Demilune Cell and Parotid Protein from Murine Oviductal Epithelium Stimulates Preimplantation Embryo Development Endocrinology, January 1, 2006; 147(1): 79 - 87. [Abstract] [Full Text] [PDF]

				J.S. Xu, Y.L. Lee, K.F. Lee, K.L. Kwok, W.M. Lee, J.M. Luk, and W.S.B. Yeung Embryotrophic factor-3 from human oviductal cells enhances proliferation, suppresses apoptosis and stimulates the expression of the {beta}1 subunit of sodium-potassium ATPase in mouse embryos Hum. Reprod., December 1, 2004; 19(12): 2919 - 2926. [Abstract] [Full Text] [PDF]

				K.-F. Lee, W. S.B. Yeung, J. F.C. Chow, C. K. Shum, and J. M. Luk Different Testicular Gene Expression Patterns in the First Spermatogenic Cycle of Postnatal and Vitamin A-Deficient Rat Testis Biol Reprod, April 1, 2004; 70(4): 1010 - 1017. [Abstract] [Full Text] [PDF]

				G. R. Bertani, C. D. Gladney, R. K. Johnson, and D. Pomp Evaluation of gene expression in pigs selected for enhanced reproduction using differential display PCR: II. Anterior pituitary J Anim Sci, January 1, 2004; 82(1): 32 - 40. [Abstract] [Full Text] [PDF]

				Y.L. Lee, K.F. Lee, J.S. Xu, K.L. Kwok, J.M. Luk, W.M. Lee, and W.S.B. Yeung Embryotrophic Factor-3 from Human Oviductal Cells Affects the Messenger RNA Expression of Mouse Blastocyst Biol Reprod, February 1, 2003; 68(2): 375 - 382. [Abstract] [Full Text] [PDF]

				D. Rizos, A. Gutierrez-Adan, S. Perez-Garnelo, J. de la Fuente, M.P. Boland, and P. Lonergan Bovine Embryo Culture in the Presence or Absence of Serum: Implications for Blastocyst Development, Cryotolerance, and Messenger RNA Expression Biol Reprod, January 1, 2003; 68(1): 236 - 243. [Abstract] [Full Text] [PDF]

				D. Rizos, P. Lonergan, M.P. Boland, R. Arroyo-Garcia, B. Pintado, J. d. l. Fuente, and A. Gutierrez-Adan Analysis of Differential Messenger RNA Expression Between Bovine Blastocysts Produced in Different Culture Systems: Implications for Blastocyst Quality Biol Reprod, March 1, 2002; 66(3): 589 - 595. [Abstract] [Full Text] [PDF]

				A. Agarwal, W. S.B. Yeung, and K.-F. Lee Cloning and characterization of the human oviduct-specific glycoprotein (HuOGP) gene promoter Mol. Hum. Reprod., February 1, 2002; 8(2): 167 - 175. [Abstract] [Full Text] [PDF]