| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular Article |
a Laboratory for Pregnancy and Newborn Research, Department of Physiology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853
ABSTRACT
We determined the effect of gestational age and labor on the regional expression of prostanoid receptor genes in baboon myometrium. Cesarean hysterectomy was performed on 15 pregnant baboons of known gestational age in the last third of pregnancy, five of them during spontaneous term labor. Expression of prostanoid receptor genes was studied using Northern blot analysis. Transcripts of similar size to the human were detected for prostanoid EP1, EP2, EP3, EP4, IP, FP, and TP receptor genes using Northern blot analysis. There were no gestational age-related changes in expression of these genes. Expression of EP1, EP3, and IP receptor RNA mRNA was significantly higher in myometrium from the fundus (compared with the lower segment), whereas EP2 gene expression was significantly lower in the fundus. Labor was associated with a reduction in the regional variation of both EP2 and IP receptor gene expression, but not EP1 and EP3 expression. Labor was also associated with an overall lower level of expression of EP2 receptor mRNA. We conclude that regional and labor-related variation in myometrial expression of prostanoid receptor genes may have a key role in primate parturition.
First decision: 15 August 2000.
1 Supported by grant HD 21350 from the National Institutes of Health. G.C.S.S. was supported by the Wellcome Trust.
2 Correspondence: Gordon C.S. Smith, University of Glasgow, Department of Obstetrics and Gynaecology, The Queen Mother's Hospital, Yorkhill, Glasgow, G3 8SH, United Kingdom. FAX: 44 141 357 3610; gcs4{at}cornell.edu
This article has been cited by other articles:
|
|
 |
|
  D. P Fischer, J. A Hutchinson, D. Farrar, P. J O'Donovan, D. F Woodward, and K. M Marshall Loss of prostaglandin F2{alpha}, but not thromboxane, responsiveness in pregnant human myometrium during labour J. Endocrinol., April 1, 2008; 197(1): 171 - 179. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. Grigsby, S. R. Sooranna, B. Adu-Amankwa, B. Pitzer, D. E. Brockman, M. R. Johnson, and L. Myatt Regional Expression of Prostaglandin E2 and F2alpha Receptors in Human Myometrium, Amnion, and Choriodecidua with Advancing Gestation and Labor Biol Reprod, August 1, 2006; 75(2): 297 - 305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Schmitz, B. A Levine, and P. W Nathanielsz Localization and steroid regulation of prostaglandin E2 receptor protein expression in ovine cervix. Reproduction, April 1, 2006; 131(4): 743 - 750. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Slater, S. Astle, N. Woodcock, J. E. Chivers, N. C.J. de Wit, S. Thornton, M. Vatish, and R. Newton Anti-inflammatory and relaxatory effects of prostaglandin E2 in myometrial smooth muscle Mol. Hum. Reprod., February 1, 2006; 12(2): 89 - 97. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Olson The Promise of Prostaglandins: Have They Fulfilled Their Potential as Therapeutic Targets for the Delay of Preterm Birth? Reproductive Sciences, October 1, 2005; 12(7): 466 - 478. [Abstract] [PDF]
|
|
|
|
|
|
S.K. Banu, J.A. Arosh, P. Chapdelaine, and M.A. Fortier Expression of Prostaglandin Transporter in the Bovine Uterus and Fetal Membranes During Pregnancy Biol Reprod, August 1, 2005; 73(2): 230 - 236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Astle, S. Thornton, and D. M. Slater Identification and localization of prostaglandin E2 receptors in upper and lower segment human myometrium during pregnancy Mol. Hum. Reprod., April 1, 2005; 11(4): 279 - 287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. K. Palliser, J. J. Hirst, G. T. Ooi, G. E. Rice, N. L. Dellios, R. M. Escalona, H. C. Parkington, and I. R. Young Prostaglandin E and F Receptor Expression and Myometrial Sensitivity at Labor Onset in the Sheep Biol Reprod, April 1, 2005; 72(4): 937 - 943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Havelock, P. Keller, N. Muleba, B. A. Mayhew, B. M. Casey, W. E. Rainey, and R. A. Word Human Myometrial Gene Expression Before and During Parturition Biol Reprod, March 1, 2005; 72(3): 707 - 719. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Arosh, S. K. Banu, S. Kimmins, P. Chapdelaine, L. A. MacLaren, and M. A. Fortier Effect of Interferon-{tau} on Prostaglandin Biosynthesis, Transport, and Signaling at the Time of Maternal Recognition of Pregnancy in Cattle: Evidence of Polycrine Actions of Prostaglandin E2 Endocrinology, November 1, 2004; 145(11): 5280 - 5293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.R. Sooranna, Y. Lee, L.U. Kim, A.R. Mohan, P.R. Bennett, and M.R. Johnson Mechanical stretch activates type 2 cyclooxygenase via activator protein-1 transcription factor in human myometrial cells Mol. Hum. Reprod., February 1, 2004; 10(2): 109 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Arosh, S. K. Banu, P. Chapdelaine, and M. A. Fortier Temporal and Tissue-Specific Expression of Prostaglandin Receptors EP2, EP3, EP4, FP, and Cyclooxygenases 1 and 2 in Uterus and Fetal Membranes during Bovine Pregnancy Endocrinology, January 1, 2004; 145(1): 407 - 417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Arosh, S. K. Banu, P. Chapdelaine, V. Emond, J. J. Kim, L. A. MacLaren, and M. A. Fortier Molecular Cloning and Characterization of Bovine Prostaglandin E2 Receptors EP2 and EP4: Expression and Regulation in Endometrium and Myometrium during the Estrous Cycle and Early Pregnancy Endocrinology, July 1, 2003; 144(7): 3076 - 3091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Cook, M. C. Shallow, D. B. Zaragoza, K. I. Anderson, and D. M. Olson Mouse Placental Prostaglandins Are Associated with Uterine Activation and the Timing of Birth Biol Reprod, February 1, 2003; 68(2): 579 - 587. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
