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Regular Article |
a Division Maternal-Fetal Medicine,
b Reproductive Biology Unit, and
c Division of Reproductive Medicine, Department of Obstetrics and Gynecology, University of Ottawa, and the Ottawa Hospital Loeb Research Institute, Ottawa Hospital, Ottawa, Ontario, Canada K1H 8L6
ABSTRACT
In this study, we have examined the expression and potential role of X-linked inhibitor of apoptosis protein (XIAP), Fas, and Fas ligand (FasL) in the regulation of apoptosis throughout placental development. Protein expression was determined by Western blot analysis and immunohistochemistry, whereas apoptotic cell death was assessed by DNA fragmentation analysis and TUNEL. The XIAP was present in trophoblast throughout placental development, but its content significantly decreased during late pregnancy, when apoptosis was maximal. The FasL content was low during early placental development but increased coincidentally to the decrease in XIAP during the third trimester. Our data also suggest that placental apoptosis is the culmination of the relative expression of these cell-death and -survival proteins, a phenomenon that is cell type-specific and dependent on cytodifferentiation and the stage of placental development. Moreover, the induction of syncytiotrophoblast apoptosis may involve the concomitant up-regulation of FasL for Fas activation and the removal of downstream inhibition of the apoptotic cascade by XIAP.
First decision: 27 October 2000.
1 Supported by a grant from the Physician Services, Inc. (PSI).
2 Correspondence: Andrée Gruslin, Division Maternal-Fetal Medicine, Department of Obstetrics, Gynecology and Newborn Care, Room 8420, Ottawa Hospital-General Campus, 501 Smyth Road, Ottawa, ON, Canada K1H 8L6. FAX: 613 737 8470; agruslin{at}ottawahospital.on.ca
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