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Biology of Reproduction 64, 1682-1688 (2001)
© 2001 Society for the Study of Reproduction, Inc.

Regular Article

Autocrine/Paracrine Action of Oxytocin in Pig Endometrium1

Jianbo Hu3,a, Tenneille E. Ludwig4,a, Ugur Sallib, Fredrick Stormshakb, and Mark A. Mirando2,a

a Department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, Washington 99164-6353 b Department of Animal Science, Oregon State University, Corvallis, Oregon 97331-6702

ABSTRACT

Luminal epithelial cells of porcine endometrium are unresponsive to oxytocin (OT) in vitro although they express the greatest quantity of OT and receptors for OT in vivo. Therefore, the objective of this study was to determine if oxytocin acted in an autocrine manner on luminal epithelial cells to stimulate prostaglandin (PG)F2{alpha} secretion. Treatment of endometrial explants or enriched luminal epithelial cells with OT antagonist L-366,948 decreased (P < 0.05) basal secretion of PGF2{alpha}. Oxytocin increased (P < 0.01) PGF2{alpha} secretion from luminal epithelial cells that were pretreated with 1:5000 or 1:500 OT antiserum for 3 h to immunoneutralize endogenously secreted OT. However, OT only increased (P < 0.05) PGF2{alpha} secretion from glandular epithelial cells when pretreated with 1:500 OT antiserum. Pretreatment with OT antiserum did not alter the ability of OT to induce PGF2{alpha} secretion from stromal cells. Medium conditioned by culture of luminal epithelial cells stimulated (P < 0.05) phospholipase C activity in stromal cells, indicative of the presence of bioactive OT. Oxytocin was secreted by luminal epithelial cells and 33% was released from the apical surface. These results indicate that luminal epithelial cells secrete OT that acts in an autocrine and/or paracrine manner in pig endometrium to stimulate PGF2{alpha} secretion.

FOOTNOTES

First decision: 20 December 2000.

1 This research was supported by NIH grant HD30268.

2 Correspondence and current address: Mark A. Mirando, National Research Initiative Competitive Grants Program, Stop 2241, 1400 Independence Ave. SW, Washington, DC 20250-2241. FAX: 202 205 3641;mmirando{at}reeusda.gov

3 Current address: Oregon Regional Primate Research Center, Beaverton, OR 97006.

4 Current address: Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, Madison, WI 53706-1581.




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