| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular Article |
a Department of Animal Science, University of Missouri-Columbia, Columbia, Missouri 65201
b BioTransplant Inc., Charlestown, Massachusetts 02129
ABSTRACT
Different factors are believed to influence the outcome of nuclear transfer (NT) experiments. Besides the cell cycle stage of both recipient cytoplast and donor karyoplast, the origin of the donor cells (embryonic, fetal, and adult) is of interest. We compared in vitro development of NT embryos derived from small serum-starved (G0) or small cycling (G1) porcine fetal fibroblast cells. Serum starvation did not have a positive effect on cleavage rate or the percentage of embryos that developed to the morula and blastocyst stages. Next, we investigated the development of porcine NT embryos derived from different transgenic clonal cell lines that had originated from the same fetus. When different clonal lines of fetal fibroblasts were fused to enucleated metaphase II oocytes, differences in fusion rates as well as in development to the morula and blastocyst stages were observed (P < 0.05). When oocytes derived from sow ovaries were used as recipient cytoplasts, significantly better cleavage (P = 0.03) and blastocyst formation (P < 0.014) was obtained when compared with oocytes derived from gilts. Our data indicate that not only different cell lines, but also different clones derived from one primary cell line, result in different development when used for NT. In addition, the use of sow oocytes as a cytoplast source also improves the efficiency of NT experiments.
First decision: 5 December 2000.
1 Supported by a grant from BioTransplant Inc., Charlestown, Massachusetts, and contribution 13089 from the Missouri Agricultural Experiment Station Journal Series.
2 Correspondence: Randall S. Prather, Department of Animal Sciences, 162 Animal Science Research Center, University of Missouri-Columbia, Columbia, MO 65211. FAX: 573 882 6827; pratherr{at}missouri.edu
This article has been cited by other articles:
|
|
 |
|
  M. Sparman, V. Dighe, H. Sritanaudomchai, H. Ma, C. Ramsey, D. Pedersen, L. Clepper, P. Nighot, D. Wolf, J. Hennebold, et al. Epigenetic Reprogramming by Somatic Cell Nuclear Transfer in Primates Stem Cells, June 1, 2009; 27(6): 1255 - 1264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Zhou, S.H. Yang, C.H. Ding, X.C. He, Y.H. Xie, T.B. Hildebrandt, S.M. Mitalipov, X.H. Tang, D.P. Wolf, and W.Z. Ji A comparative approach to somatic cell nuclear transfer in the rhesus monkey Hum. Reprod., October 1, 2006; 21(10): 2564 - 2571. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Lagutina, G. Lazzari, R. Duchi, S. Colleoni, N. Ponderato, P. Turini, G. Crotti, and C. Galli Somatic cell nuclear transfer in horses: effect of oocyte morphology, embryo reconstruction method and donor cell type Reproduction, October 1, 2005; 130(4): 559 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhu, J. A. Craig, P. W. Dyce, N. Sunnen, and J. Li Embryos Derived from Porcine Skin-Derived Stem Cells Exhibit Enhanced Preimplantation Development Biol Reprod, December 1, 2004; 71(6): 1890 - 1897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.M. Powell, N.C. Talbot, K.D. Wells, D.E. Kerr, V.G. Pursel, and R.J. Wall Cell Donor Influences Success of Producing Cattle by Somatic Cell Nuclear Transfer Biol Reprod, July 1, 2004; 71(1): 210 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hyun, G. Lee, D. Kim, H. Kim, S. Lee, D. Nam, Y. Jeong, S. Kim, S. Yeom, S. Kang, et al. Production of Nuclear Transfer-Derived Piglets Using Porcine Fetal Fibroblasts Transfected with the Enhanced Green Fluorescent Protein Biol Reprod, September 1, 2003; 69(3): 1060 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Miyoshi, S. J. Rzucidlo, S. L. Pratt, and S. L. Stice Improvements in Cloning Efficiencies May Be Possible by Increasing Uniformity in Recipient Oocytes and Donor Cells Biol Reprod, April 1, 2003; 68(4): 1079 - 1086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Booth, D. Viuff, S. Tan, P. Holm, T. Greve, and H. Callesen Numerical Chromosome Errors in Day 7 Somatic Nuclear Transfer Bovine Blastocysts Biol Reprod, March 1, 2003; 68(3): 922 - 928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Arat, J. Gibbons, S. J. Rzucidlo, D. S. Respess, M. Tumlin, and S. L. Stice In Vitro Development of Bovine Nuclear Transfer Embryos from Transgenic Clonal Lines of Adult and Fetal Fibroblast Cells of the Same Genotype Biol Reprod, June 1, 2002; 66(6): 1768 - 1774. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
