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Regular Article |
1 Isoforms of the Catalytic Subunit of Cyclic 3',5'-Adenosine Monophosphate-Dependent Protein Kinase Testicular Cells1
a Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655
ABSTRACT
The amino terminus of the sperm cAMP-dependent protein kinase catalytic subunit (termed Cs) differs from that of the C
1 isoform expressed in most tissues due to the use of alternative transcripts of the C gene. Both C1 and Cs transcripts are present in testis; Cs is expressed specifically in spermatogenic cells and is the only C isoform detected in mature sperm. Immunohistochemistry of mouse testis using antibodies specific for Cs and C1 now shows that C1 is present in somatic testicular cells, spermatogonia, and preleptotene spermatocytes but not in cells that are in later stages of spermatogenesis. In contrast, Cs is expressed only in midpachytene and later stage spermatocytes and in spermatids. Therefore, Cs and C1 expression do not overlap. Immunofluorescence microscopic localization of Cs in murine and ovine sperm reveals that Cs is located primarily in sperm tail components, including the midpiece mitochondria and the axoneme. Quantitative analysis of Western blots indicates that individual ovine sperm contain 
4 x 105 molecules of Cs, a seemingly large number for a protein that acts catalytically.
First decision: 4 December 2000.
1 Supported by National Institutes of Health grant HD23858 and by the Robert W. Booth Fund at the Greater Worcester Community Foundation.
2 Correspondence: George Witman, Department of Cell Biology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655. FAX: 508 856 5612; george.witman{at}umassmed.edu
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