| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular Article |
a Department of Clinical Studies, Reproduction,
b Department of Mathematics and Physics, and
c Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, 1870 Frederiksberg C, Denmark Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands
ABSTRACT
The frequency of chromosome abnormalities was investigated in cattle embryos (n = 256) derived from superovulated heifers (n = 35) on Days 2, 3, 4, and 5 postovulation (PO). Interphase nuclei (n = 4358) were analyzed for chromosome abnormalities using fluorescent in situ hybridization with chromosome 6- and chromosome 7-specific probes and the developmental rate was described by scoring cell numbers. We found that 93%, 85%, 84%, and 69% of the embryos from Days 2, 3, 4, and 5 PO, respectively, displayed a normal diploid chromosome number in all cells. Of the embryos containing abnormal cells, mixoploidy was significantly more frequent than polyploidy. The percentage of mixoploidy at Days 2, 3, 4, and 5 PO was 5%, 13%, 16%, and 31%, respectively, whereas the percentages of polyploidy were 2%, 2%, 0%, and 0%, respectively. The mean number of cells per embryo was 4.7, 8, 11.5, and 48.3, respectively, at Days 2, 3, 4, and 5 PO. Thus, in vivo-developed embryos were significantly more advanced than the in vitro-produced (IVP) embryos except for Day 2. In conclusion, a significantly lower frequency of chromosomally abnormal embryos, in particular displaying polyploidy early after fertilization, was seen in in vivo versus IVP embryos, and these chromosomal abnormalities may be inherent to the process of IVP in cattle.
First decision: 5 February 2001.
1 Supported by the Danish Agricultural and Veterinary Research Council, Danish Biotechnology Program and Novo Nordisk A/S. P.J.M.H. is supported by Holland Genetics (Arnhem, The Netherlands) and the European Union.
2 Correspondence: Dorthe Viuff, Department of Clinical Studies, Reproduction, Royal Veterinary and Agricultural University, Dyrlægevej 68, DK-1870 Frederiksberg C, Denmark. FAX: 45 35 28 29 72; dv{at}kvl.dk
This article has been cited by other articles:
|
|
 |
|
  N. I Alexopoulos, P. Maddox-Hyttel, P. Tveden-Nyborg, N. T D'Cruz, T. R Tecirlioglu, M. A Cooney, K. Schauser, M. K Holland, and A. J French Developmental disparity between in vitro-produced and somatic cell nuclear transfer bovine days 14 and 21 embryos: implications for embryonic loss Reproduction, October 1, 2008; 136(4): 433 - 445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Mateusen, A. Van Soom, D. G D Maes, I. Donnay, L. Duchateau, and A.-S. Lequarre Porcine embryo development and fragmentation and their relation to apoptotic markers: a cinematographic and confocal laser scanning microscopic study Reproduction, April 1, 2005; 129(4): 443 - 452. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Lonergan, H. G. Pedersen, D. Rizos, T. Greve, P. D. Thomsen, T. Fair, A. Evans, and M. P. Boland Effect of the Post-Fertilization Culture Environment on the Incidence of Chromosome Aberrations in Bovine Blastocysts Biol Reprod, October 1, 2004; 71(4): 1096 - 1100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. O. Gjorret, H. M. Knijn, S. J. Dieleman, B. Avery, L.-I. Larsson, and P. Maddox-Hyttel Chronology of Apoptosis in Bovine Embryos Produced In Vivo and In Vitro Biol Reprod, October 1, 2003; 69(4): 1193 - 1200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. M. Knijn, J. O. Gjorret, P. L.A.M. Vos, P. J.M. Hendriksen, B. C. van der Weijden, P. Maddox-Hyttel, and S. J. Dieleman Consequences of In Vivo Development and Subsequent Culture on Apoptosis, Cell Number, and Blastocyst Formation in Bovine Embryos Biol Reprod, October 1, 2003; 69(4): 1371 - 1378. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
