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Transgenic Major Histocompatibility Complex Class I Antigen Expressed in Mouse Trophoblast Affects Maternal Immature B Cells¹

^a Laboratory of Immune Regulations and Development, ^b Department of Developmental Biology, and Flow Cytometry Unit, J. Monod Institute, UMR 7592 (CNRS and Universities Paris 6 and 7), 75251 Paris cedex 05, France

ABSTRACT

We have produced transgenic mice using the mouse placental lactogen type II promoter to force and restrict the expression of the mouse major histocompatibility complex (MHC) class I molecule, H-2K^b, to the placenta. We show that the transgenic MHC antigen H-2K^b is expressed exclusively in trophoblast giant cells from Day 10.5 until the end of gestation. This expression affects neither the fetal development nor the maternal tolerance to the fetus in histoincompatible mothers. We have used the 3.83 B cell receptor (BcR) transgenic mouse line to follow the fate of H-2K^b-specific maternal B cells in mothers bearing H-2K^b-positive placentas. Our results suggest that transgenic H-2K^b molecules on trophoblast giant cells are recognized by 3.83 BcR-transgenic B cells in the bone marrow of pregnant females. This antigen recognition triggers the deletion of a bone marrow B cell subpopulation, including immature and transitional B cells. Their percentage decreases during the second half of gestation and is down to 8% on Day 17.5, compared to 22% in the (3.83 Tg female x Fvb) control group. This deletion might contribute to the process of maternal tolerance of the conceptus.

FOOTNOTES

First decision: 16 February 2001.

¹ This work has been supported in part by INSERM, the Association pour la Recherche contre le Cancer (ARC) and the Fondation pour la Recherche Médicale. D.A.-A. received a fellowship from the Fondation pour la Recherche Médicale and A.L. was supported by the European Community.

² Correspondence: Colette Kanellopoulos-Langevin, Laboratory of Immune Regulations and Development, J. Monod Institute, Tour 43, 2 place Jussieu, 75251 Paris cedex 05, France. FAX: 331 44275265; kanellopoulos{at}ijm.jussieu.fr

³ Current address: Scripps Research Institute, La Jolla, CA 92037.

⁴ Current address: Applied Biosystems, Grundstrasse 10, CH-6343 Rotkreuz, Switzerland.

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