| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular Article |
a Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo 162-8640, Japan
b Tsukuba Primate Center, National Institute of Infectious Diseases, Ibaraki 305-0843, Japan
c Laboratory Animal Research Center, University of Tsukuba, Ibaraki 305-8575, Japan
d Department of Physiology, Tokyo Women's Medical University of Medicine, Tokyo 162-8666, Japan
e Laboratory of Intracellular Metabolism, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
ABSTRACT
It is widely accepted that mature mammalian oocytes are induced to resume meiosis by a sperm-borne oocyte-activating factor(s) (sperm factor, SF) immediately after normal fertilization or intracytoplasmic sperm injection. The SF is most likely a soluble factor that is localized within the cytoplasm of mature spermatozoa, but the exact stage at which it appears during spermatogenesis and its localization after oocyte activation is not fully understood, except in the mouse. First, we injected mature spermatozoa and spermatogenic cells from cynomolgus monkeys into mouse oocytes to assess their oocyte-activating capacity. More than 90% of mouse oocytes were activated after injection of monkey spermatozoa. Round spermatids and primary spermatocytes (late pachytene to diplotene) also activated oocytes (93% and 79%, respectively). Injection of monkey spermatozoa and spermatids induces intracellular Ca2+ oscillations in a pattern similar to that seen following normal fertilization. Most spermatocytes did not produce typical intracellular Ca2+ oscillations. Second, we transferred pronuclei or cytoplasts from mouse oocytes that had been activated by monkey spermatozoa or spermatids into intact mature mouse oocytes by electrofusion in order to examine the localization of the SF after pronuclear formation. Some of the SF was localized within the pronuclei, but some stayed in the ooplasm. This study demonstrated that spermatogenic cells of cynomolgus monkeys acquire oocyte-activating capacity at much earlier stages than those of mice, and that the monkey SF has a pronucleus-directing nature, although to a lesser extent than the mouse SF.
First decision: 22 January 2001.
1 Supported by grants from the Ministry of Education, Science, Sports, and Culture, Japan, and the Ministry of Health and Welfare, Japan.
2 Correspondence: Atsuo Ogura, Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1, Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. FAX: 81 3 5285 1111; aogura{at}nih.go.jp
This article has been cited by other articles:
|
|
 |
|
  M. Ito, T. Shikano, S. Oda, T. Horiguchi, S. Tanimoto, T. Awaji, H. Mitani, and S. Miyazaki Difference in Ca2+ Oscillation-Inducing Activity and Nuclear Translocation Ability of PLCZ1, an Egg-Activating Sperm Factor Candidate, Between Mouse, Rat, Human, and Medaka Fish Biol Reprod, June 1, 2008; 78(6): 1081 - 1090. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-Y. Yoon and R. A Fissore Release of phospholipase C {zeta}and [Ca2+]i oscillation-inducing activity during mammalian fertilization Reproduction, November 1, 2007; 134(5): 695 - 704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ogonuki, H. Tsuchiya, Y. Hirose, H. Okada, A. Ogura, and T. Sankai Pregnancy by the tubal transfer of embryos developed after injection of round spermatids into oocyte cytoplasm of the cynomolgus monkey (Macaca fascicularis) Hum. Reprod., June 1, 2003; 18(6): 1273 - 1280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ogonuki, K. Mochida, K. Inoue, J. Matsuda, Y. Yamamoto, K. Takano, and A. Ogura Fertilization of Oocytes and Birth of Normal Pups Following Intracytoplasmic Injection with Spermatids in Mastomys (Praomys coucha) Biol Reprod, May 1, 2003; 68(5): 1821 - 1827. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
