| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular Article |
a Department of Anesthesiology, College of Physicians & Surgeons, Columbia University, New York, New York 10032
b Westfälische Wilhelms-Universität, Münster 4400, Germany
c The Johns Hopkins Institutions, Baltimore, Maryland 21287-7294
ABSTRACT
The actin cytoskeleton is important for stress fiber formation and contributes to the initiation and maintenance of smooth muscle contraction. To determine if oxytocin and lysophosphatidic acid (LPA) induce stress fiber formation, cultured human myometrial cells were exposed to oxytocin (10-5 M) or LPA (10-6 M), and filamentous (F) and globular (G) actin pools were stained with fluorescein isothiocyanate-phalloidin and Texas red DNase I, respectively. The F- to G-actin fluorescent-staining ratio was measured by fluorescence microscopy. Oxytocin and LPA increased stress fiber formation, as indicated by an increase in the F- to G-actin fluorescent-staining ratio. The Rho-kinase inhibitor Y-27632 markedly attenuated this increase. Oxytocin-induced stress fiber formation was completely inhibited in the presence of the oxytocin antagonist compound VI. Tyrosine kinase inhibition with tyrphostin A23 partially blocked the increase induced by oxytocin but had no effect on LPA-induced stress fiber formation. Stress fiber formation was not blocked by pertussis toxin, mitogen-activated protein kinase, or protein kinase C inhibitors. Our results show that human myometrial cells respond to oxytocin and LPA with the formation of stress fibers that may be involved in the maintenance of uterine contractions. Rho-kinase appears to be a key signaling factor in this pathway.
First decision: 27 November 2000.
1 The National Institutes of Health NHLBI RO1 HL 62340 in part supported this study. W.G. is a postdoctoral fellow supported by the Innovative Medizinische Forschung, Germany.
2 Correspondence: Carol A. Hirshman, Department of Anesthesiology, College of Physicians & Surgeons of Columbia University, P & S Box 46, 630 West 168th Street, New York, NY 10032. FAX: 212 305 8980; cah63{at}columbia.edu
This article has been cited by other articles:
|
|
 |
|
  X. Ye Lysophospholipid signaling in the function and pathology of the reproductive system Hum. Reprod. Update, September 1, 2008; 14(5): 519 - 536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Garcia-Verdugo, Z. Tanfin, E. Dallot, M.-J. Leroy, and M. Breuiller-Fouche Surfactant Protein A Signaling Pathways in Human Uterine Smooth Muscle Cells Biol Reprod, August 1, 2008; 79(2): 348 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Billon-Denis, Z. Tanfin, and P. Robin Role of lysophosphatidic acid in the regulation of uterine leiomyoma cell proliferation by phospholipase D and autotaxin J. Lipid Res., February 1, 2008; 49(2): 295 - 307. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Wu, H. Shen, L. Yu, M. Peng, W.-S. Lai, and Y.-L. Ding Corticotropin-releasing hormone activates connexin 43 via activator protein-1 transcription factor in human myometrial smooth muscle cells Am J Physiol Endocrinol Metab, December 1, 2007; 293(6): E1789 - E1794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-J. Jeng, V. R. Suarez, M. G. Izban, H.-Q. Wang, and M. S. Soloff Progesterone-induced sphingosine kinase-1 expression in the rat uterus during pregnancy and signaling consequences Am J Physiol Endocrinol Metab, April 1, 2007; 292(4): E1110 - E1121. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-F. Wang and G. I. Hatton Mechanisms Underlying Oxytocin-Induced Excitation of Supraoptic Neurons: Prostaglandin Mediation of Actin Polymerization J Neurophysiol, June 1, 2006; 95(6): 3933 - 3947. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Breuiller-Fouche and G. Germain Gene and protein expression in the myometrium in pregnancy and labor. Reproduction, May 1, 2006; 131(5): 837 - 850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Soloff, Y.-J. Jeng, M. Ilies, S. L. Soloff, M. G. Izban, T. G. Wood, N. I. Panova, G. V.N. Velagaleti, and G. D. Anderson Immortalization and characterization of human myometrial cells from term-pregnant patients using a telomerase expression vector Mol. Hum. Reprod., September 1, 2004; 10(9): 685 - 695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Gogarten, K. S. Lindeman, C. A. Hirshman, and C. W. Emala Tumor Necrosis Factor Alpha Stimulates Adenylyl Cyclase Activity in Human Myometrial Cells Biol Reprod, March 1, 2003; 68(3): 751 - 757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Dallot, M. Pouchelet, N. Gouhier, D. Cabrol, F. Ferre, and M. Breuiller-Fouche Contraction of Cultured Human Uterine Smooth Muscle Cells after Stimulation with Endothelin-1 Biol Reprod, March 1, 2003; 68(3): 937 - 942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. T. Budnik and A. K. Mukhopadhyay Lysophosphatidic Acid and Its Role in Reproduction Biol Reprod, April 1, 2002; 66(4): 859 - 865. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
