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Regular Article |
4,5 Isomerase Compared to Normal Dominant Follicles
a Departments of Animal Sciences,
b Veterinary Medicine and Surgery,
c Biochemistry and Child Health, University of Missouri-Columbia, Columbia, Missouri 65211-5300
ABSTRACT
The objective was to compare ovarian steroids and expression of mRNAs encoding cytochrome P450 side-chain cleavage, cytochrome P450 17
-hydroxylase, cytochrome P450 aromatase, 3ß-hydroxysteroid dehydrogenase 
4,5 isomerase, LH, and FSH receptors and estrogen receptor-ß in ovaries of cows with dominant and nondominant ovarian follicular cysts and in normal dominant follicles. Estradiol-17ß, progesterone, and androstenedione concentrations were determined in follicular fluid using specific RIAs. Dominant cysts were larger than young cysts or dominant follicles, whereas nondominant cysts were intermediate. Estradiol-17ß (ng/ml) and total steroids (ng/follicle) were higher in dominant cysts than in dominant follicles. Expression of LH receptor and 3ß-hydroxysteroid dehydrogenase mRNAs was higher in granulosa cells of dominant cysts than in dominant follicles. Nondominant cysts had higher follicular concentrations of progesterone, lower estradiol-17ß concentrations, and lower expression of steroidogenic enzyme, gonadotropin receptor, and estrogen receptor-ß mRNAs than other groups. In summary, increased expression of LH receptor and 3ß-hydroxysteroid dehydrogenase mRNAs in granulosa and increased follicular estradiol-17ß concentrations were associated with dominant cysts compared to dominant follicles. Study of cysts at known developmental stages is useful in identifying alterations in follicular steroidogenesis.
First decision: 18 October 2000.
1 Correspondence: H. Allen Garverick, Department of Animal Sciences, 163 Animal Science Research Center, 920 E. Campus Dr., University of Missouri-Columbia, Columbia, MO 65211-5300. FAX: 573 882 6827;garvericka{at}missouri.edu
2 Current address: Departments of Obstetrics and Gynecology and Physiology, University of Western Ontario, London, ON, Canada N6A 5C1.
3 Current address: Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-5602.
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