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Mares with Delayed Uterine Clearance Have an Intrinsic Defect in Myometrial Function¹

^a Department of Large Animal Medicine & Surgery, ^b Department of Anatomy & Public Health, ^c College of Veterinary Medicine, and Department of Health & Kinesiology, College of Education, Texas A&M University, College Station, Texas 77843-4475

ABSTRACT

Persistent, postmating endometritis affects approximately 15% of mares and results in reduced fertility and sizable economic losses to the horse-breeding industry. Mares that are susceptible to postmating endometritis have delayed uterine clearance associated with reduced uterine contractility. Unfortunately, the mechanism for reduced uterine contractility remains an enigma. The present study examined the hypothesis that mares with delayed uterine clearance have an intrinsic contractile defect of the myometrium. Myometrial contractility was evaluated in vitro by measuring isometric tension generated by longitudinal and circular uterine muscle strips in response to KCl, oxytocin, and prostaglandin F₂ (PGF₂) for young nulliparous mares, older reproductively normal mares, and older mares with delayed uterine clearance. In addition, intracellular Ca²⁺ regulation was evaluated using laser cytometry to measure oxytocin-stimulated intracellular Ca²⁺ transients of myometrial cells loaded with a Ca²⁺-sensitive fluorescent dye, fluo-4. For all contractile agonists, myometrium from mares with delayed uterine clearance failed to generate as much tension as myometrium from older normal mares. Oxytocin-stimulated intracellular Ca²⁺ transients were similar for myometrial cells from mares with delayed uterine clearance and from older normal mares, suggesting that the contractile defect did not result from altered regulation of intracellular Ca²⁺ concentration. Furthermore, no apparent age-dependent decline was observed in myometrial contractility; KCl-depolarized and oxytocin-stimulated longitudinal myometrium from young normal mares and older normal mares generated similar responses. However, circular myometrium from young normal mares failed to generate as much tension as myometrium from older normal mares when stimulated with oxytocin or PGF₂, suggesting possible age-related alterations in receptor-second messenger signaling mechanisms downstream of intracellular Ca²⁺ release. In summary, for mares with delayed uterine clearance, an intrinsic contractile defect of the myometrium may contribute to reduced uterine contractility following breeding.

FOOTNOTES

First decision: 20 December 2000.

¹ Supported by the Grayson-Jockey Club Research Foundation, the Texas Equine Research Fund, and a USDA-Animal Health Formula grant. Preliminary results from this study were presented at the Annual Conference of the Society for Theriogenology, September 1999, Nashville, TN.

² Correspondence. FAX: 979 847 8863; srigby{at}cvm.tamu.edu