HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chaffin, C. L. Articles by Stouffer, R. L. Search for Related Content PubMed PubMed Citation Articles by Chaffin, C. L. Articles by Stouffer, R. L. Agricola Articles by Chaffin, C. L. Articles by Stouffer, R. L.

Gonadotropin and Steroid Control of Granulosa Cell Proliferation During the Periovulatory Interval in Rhesus Monkeys¹

^a Division of Reproductive Sciences, Oregon Regional Primate Research Center, Beaverton, Oregon 97006 ^b Department of Physiology and Pharmacology, Oregon Health Sciences University, Portland, Oregon 97201

ABSTRACT

Progesterone produced in response to the midcycle gonadotropin surge is essential for ovulation and luteinization of the primate follicle. Because cell-cycle arrest is associated with the initiation of luteinization, this study was designed to determine the dynamics and regulation of granulosa cell proliferation by gonadotropin and progesterone during the periovulatory interval in the primate follicle. Granulosa cells or ovaries were obtained from macaques undergoing controlled ovarian stimulation either before (0 h) or as long as 36 h following the administration of an ovulatory hCG bolus with or without a 3ß-hydroxysteroid dehydrogenase inhibitor with or without a nonmetabolizable progestin. The percentage of cells staining positive for Ki-67, a nuclear marker for cell proliferation, decreased (P < 0.05) within 12 h of hCG administration in a steroid-independent manner. Levels of cyclin D2 and E mRNA did not decline during the periovulatory interval; however, cyclin B1 mRNA was reduced significantly by 12 h. Steroid depletion increased (P < 0.05) cyclin B1 mRNA at both 12 and 36 h post-hCG and was reversible by progestin replacement at 36 h. The cyclin-dependent kinase inhibitor p21^Cip1 was transiently increased 12 h post-hCG, whereas p27^Kip1 mRNA levels increased at 36 h in a steroid-independent fashion. These data suggest that a gonadotropin bolus inhibits mitosis in granulosa cells early (12 h) in the periovulatory interval, whereas progesterone may play a later, antiproliferative role in luteinized cells of primates.

FOOTNOTES

First decision: 25 January 2001.

¹ Supported by NIH/NICHD HD20869, U54 HD18185 as part of the Specialized Cooperative Centers Program in Reproductive Research, HD8302, and RR00163.

² Correspondence: Richard L. Stouffer, Division of Reproductive Sciences, Oregon Regional Primate Research Center, 505 NW 185th St., Beaverton, OR 97006. FAX: 503 690 5563; stouffri{at}ohsu.edu

³ Current address: Department of Physiology, Medical College of Georgia, Augusta, GA 30912.

This article has been cited by other articles:

				M. B. Stanek, S. M. Borman, T. A. Molskness, J. M. Larson, R. L. Stouffer, and P. E. Patton Insulin and Insulin-Like Growth Factor Stimulation of Vascular Endothelial Growth Factor Production by Luteinized Granulosa Cells: Comparison between Polycystic Ovarian Syndrome (PCOS) and Non-PCOS Women J. Clin. Endocrinol. Metab., July 1, 2007; 92(7): 2726 - 2733. [Abstract] [Full Text] [PDF]

				K.N. Fru, M. Cherian-Shaw, M. Puttabyatappa, C.A. VandeVoort, and C.L. Chaffin Regulation of granulosa cell proliferation and EGF-like ligands during the periovulatory interval in monkeys Hum. Reprod., May 1, 2007; 22(5): 1247 - 1252. [Abstract] [Full Text] [PDF]

				S. M Quirk, R. G Cowan, and R. M Harman The susceptibility of granulosa cells to apoptosis is influenced by oestradiol and the cell cycle. J. Endocrinol., June 1, 2006; 189(3): 441 - 453. [Abstract] [Full Text] [PDF]

				J. D. Cannon, M. Cherian-Shaw, and C. L. Chaffin Proliferation of Rat Granulosa Cells during the Periovulatory Interval Endocrinology, January 1, 2005; 146(1): 414 - 422. [Abstract] [Full Text] [PDF]

				M. Cherian-Shaw, R. Das, C. A. VandeVoort, and C. L. Chaffin Regulation of Steroidogenesis by p53 in Macaque Granulosa Cells and H295R Human Adrenocortical Cells Endocrinology, December 1, 2004; 145(12): 5734 - 5744. [Abstract] [Full Text] [PDF]

				S. M. Quirk, R. G. Cowan, and R. M. Harman Progesterone Receptor and the Cell Cycle Modulate Apoptosis in Granulosa Cells Endocrinology, November 1, 2004; 145(11): 5033 - 5043. [Abstract] [Full Text] [PDF]

				S. M. Borman, C. L. Chaffin, K. M. Schwinof, R. L. Stouffer, and M. B. Zelinski-Wooten Progesterone Promotes Oocyte Maturation, but Not Ovulation, in Nonhuman Primate Follicles Without a Gonadotropin Surge Biol Reprod, July 1, 2004; 71(1): 366 - 373. [Abstract] [Full Text] [PDF]

				S. M. Quirk, R. G. Cowan, R. M. Harman, C.-L. Hu, and D. A. Porter Ovarian follicular growth and atresia: The relationship between cell proliferation and survival J Anim Sci, January 1, 2004; 82(13_suppl): E40 - 52. [Abstract] [Full Text] [PDF]

				C. L. Chaffin, R. S. Brogan, R. L. Stouffer, and C. A. VandeVoort Dynamics of Myc/Max/Mad Expression during Luteinization of Primate Granulosa Cells in Vitro: Association with Periovulatory Proliferation Endocrinology, April 1, 2003; 144(4): 1249 - 1256. [Abstract] [Full Text] [PDF]

				C. Gillio-Meina, Y. Y. Hui, and H. A. LaVoie GATA-4 and GATA-6 Transcription Factors: Expression, Immunohistochemical Localization, and Possible Function in the Porcine Ovary Biol Reprod, February 1, 2003; 68(2): 412 - 422. [Abstract] [Full Text] [PDF]