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Biology of Reproduction 65, 771-776 (2001)
© 2001 Society for the Study of Reproduction, Inc.

Regular Article

Nature of the Spermatogenic Arrest in Dazl -/- Mice

Bianca H.G.J. Schrans-Stassena, Philippa T.K. Saundersb, Howard J. Cookec, and Dirk G. de Rooija

a Department of Cell Biology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands b Medical Research Council Human Reproductive Sciences Unit, Edinburgh EH3 9ET, United Kingdom c Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, United Kingdom

ABSTRACT

Dazl encodes an RNA-binding protein essential for spermatogenesis. Mice that are deficient for Dazl are infertile, lacking any formation of spermatozoa, and the only germ cells present are spermatogonia and a few spermatocytes. To gain more insight regarding the timing of the spermatogenic arrest in Dazl -/- mice, we studied the spermatogonial cell types present in testis sections and in seminiferous tubular whole mounts. Most of the seminiferous tubular cross-sections contained A spermatogonia as the most advanced cell type, with only very few containing cells up to pachytene spermatocytes. Both 5-bromodeoxy-uridine incorporation and mitotic index indicated that the remaining A spermatogonia were actively proliferating. C-kit immunohistochemical studies showed that most of the A spermatogonia were positively stained for the c-Kit protein (~80%). The clonal composition of the A spermatogonia in tubular whole mounts indicated these cells to be Asingle (As), Apaired (Apr), and Aaligned (Aal) spermatogonia. It is concluded that the prime spermatogenic defect in the Dazl -/- mice is a failure of the great majority of the Aal spermatogonia to differentiate into A1 spermatogonia. As a result, most seminiferous tubules of Dazl -/- mice only contain actively proliferating As, Apr, and Aal spermatogonia, with cell production being equaled by apoptosis of these cells.

FOOTNOTES

First decision: 25 October 2000.

1 Correspondence: Dirk G. de Rooij, Department of Cell Biology, University Medical Center Utrecht, AZU-Rm G02.525, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands. FAX: 31 0 30 2541797; d.g.derooij{at}med.uu.nl




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