HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Coya, R. Articles by María Señarís, R. Search for Related Content PubMed PubMed Citation Articles by Coya, R. Articles by María Señarís, R. Agricola Articles by Coya, R. Articles by María Señarís, R.

Effect of Cyclic 3',5'-Adenosine Monophosphate, Glucocorticoids, and Insulin on Leptin Messenger RNA Levels and Leptin Secretion in Cultured Human Trophoblast¹

^a Hormone Laboratory, ^b Section of Pediatric Endocrinology, Hospital de Cruces, Barakaldo, 48903 País Vasco, Spain ^c Department of Biochemistry and Molecular Biology, Universidad del País Vasco, 48903 País Vasco, Spain ^d Department of Physiology, Universidad de Santiago de Compostela, 15705 Santiago de Compostela, Spain

ABSTRACT

Leptin is a polypeptide hormone originally thought to be produced exclusively by adipocytes. However, both leptin mRNA and leptin protein were identified in human placental trophoblast cells, suggesting a potential role in human pregnancy. In the present report, we examined the regulation of leptin mRNA levels and secretion by cAMP, glucocorticoids, and insulin in term human placental tissue. Placentae were obtained immediately after delivery from mothers with uncomplicated pregnancies. Leptin concentrations were measured by ELISA in the cultured media of trophoblast maintained in monolayer culture for 24, 48, and 72 h. Likewise leptin mRNA levels in these cultured human trophoblast cells were determined by reverse transcription-polymerase chain reaction. Treatment with forskolin and (Bu)₂ cAMP led to a time- and dose-dependent increase in leptin release, significant after 48 and 72 h. Moreover, incubation with forskolin for 48 h also clearly increased leptin mRNA concentration. Leptin secretion and mRNA levels were also assessed after treatment with insulin or dexamethasone. We found a time- and dose-dependent increase in leptin release, significant after 48 and 72 h. Leptin mRNA levels were also increased after these treatments. All this supports a stimulatory role of cAMP pathway, insulin and dexamethasone in the leptin mRNA levels, and leptin release in trophoblast cells in vitro.

FOOTNOTES

First decision: 12 December 2000.

¹ This work was supported by Fondo de Investigación Sanitaria (FISS 99/0678), Spanish Ministry of Health, and Lilly, S.A. (Spain).

² Correspondence: Rosa María Señarís, Department of Physiology, Faculty of Medicine, C/ San Francisco s/n, 15705 Santiago de Compostela, Spain. FAX: 34 981 574145; fsrsr{at}usc.es

This article has been cited by other articles:

				M. C. Henson and V. D. Castracane Leptin in Pregnancy: An Update Biol Reprod, February 1, 2006; 74(2): 218 - 229. [Abstract] [Full Text] [PDF]

				M C Henson, K F Swan, D E Edwards, G W Hoyle, J Purcell, and V D Castracane Leptin receptor expression in fetal lung increases in late gestation in the baboon: a model for human pregnancy Reproduction, January 1, 2004; 127(1): 87 - 94. [Abstract] [Full Text] [PDF]

				J. Zhao, T. H. Kunz, N. Tumba, L. Clamon Schulz, C. Li, M. Reeves, and E. P. Widmaier Comparative analysis of expression and secretion of placental leptin in mammals Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2003; 285(2): R438 - R446. [Abstract] [Full Text] [PDF]

				P. Cameo, P. Bischof, and J. C. Calvo Effect of Leptin on Progesterone, Human Chorionic Gonadotropin, and Interleukin-6 Secretion by Human Term Trophoblast Cells in Culture Biol Reprod, February 1, 2003; 68(2): 472 - 477. [Abstract] [Full Text] [PDF]