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Role of Tumor Necrosis Factor in Preovulatory Follicles of Swine

^a Institute of Anatomy, Ernst Moritz Arndt University, 17487 Greifswald, Germany

ABSTRACT

The effects of tumor necrosis factor (TNF) on cultured porcine granulosa cells that were obtained from preovulatory follicles were studied with regard to following parameters: 1) TNF receptor type I expression, 2) progesterone receptor and transforming growth factor ß receptor type II (TßR II) as markers of luteinization, 3) proliferation, and 4) apoptosis. For comparative purposes the effects of TNF were also studied on insulin/forskolin-treated cells, as this treatment is well established to induce luteinization. Cytochemical methods followed by semiquantitative analysis were used. Our data show that TNF treatment upregulates TNF receptor type I expression in granulosa cells. TNF downregulates the expression of TßR II of insulin/forskolin-stimulated and of unstimulated cells. The progesterone receptor is also downregulated by the cytokine after insulin/forskolin-induced luteinization. Supplementation of the medium with TNF leads to increased proliferation and at the same time it induces apoptosis. Our results indicate that TNF exerts an inhibitory influence on luteinization and that TNF influences the balance between follicular growth (proliferation) and atresia (apoptosis).

FOOTNOTES

First decision: 31 January 2001.

¹ Correspondence: Janine Prange-Kiel, Institute of Anatomy, Ernst Moritz Arndt University, Friedrich-Loeffler-Straße 23c, 17487 Greifswald, Germany. FAX: 49 3834 865302; prange{at}mail.uni-greifswald.de

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