HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Glister, C. Articles by Knight, P. G. Search for Related Content PubMed PubMed Citation Articles by Glister, C. Articles by Knight, P. G. Agricola Articles by Glister, C. Articles by Knight, P. G.

Interactions Between Follicle-Stimulating Hormone and Growth Factors in Modulating Secretion of Steroids and Inhibin-Related Peptides by Nonluteinized Bovine Granulosa Cells¹

^a School of Animal and Microbial Sciences, The University of Reading, Whiteknights, Reading RG6 6AJ, United Kingdom ^b School of Biological and Molecular Sciences, Oxford Brookes University, Headington, Oxford OX3 0BP, United Kingdom

ABSTRACT

The aim was to investigate potential interactions between FSH and intraovarian growth factors in modulating secretion of inhibin A (inh A), activin A (act A), follistatin (FS), estradiol (E₂), and progesterone (P₄) by bovine granulosa cells cultured under conditions in which a nonluteinized FSH-responsive phenotype is maintained. Cells from 4- to 6-mm follicles were cultured in serum-free medium containing insulin (10 ng/ml) and androstenedione (10^-7 M), and effects of ovine FSH (0.037–3 ng/ml) were tested alone and in combination with insulin-like growth factors (IGF) (LR3 IGF-I analogue; 2–50 ng/ml) and epidermal growth factor (EGF; 0.1–10 ng/ml). Medium was changed every 48 h and cultures ended after 144 h, when cell number was determined. Between 48–96 h and 96–144 h, FSH promoted (P < 0.0001) increases in output of inh A (6-fold), act A (15-fold), FS (6-fold), and E₂ (18-fold), with maximal responses (in parentheses) elicited by 0.33 ng/ml FSH during the final period. Higher FSH doses (1 and 3 ng/ml) gave reduced responses for each of the above hormones, whereas P₄ output was maximal (3-fold) at these doses. FSH promoted a slight increase in cell number (1.7-fold; P < 0.001). LR3 IGF-I alone markedly increased (P < 0.0001) output of inh A (8-fold), act A (41-fold), FS (12-fold), and E₂ (18-fold); this was accompanied by modest increases (P < 0.01) in P₄ output (2.5-fold) and cell number (2-fold). Whereas FSH enhanced inh A, act A, FS, and E₂ secretion evoked by lower doses of LR3 IGF-I, it suppressed (P < 0.001) the response to the highest dose. EGF alone promoted a 1.7-fold increase in cell number (P < 0.001) without affecting hormone release; however, it abolished (P < 0.001) FSH-induced secretion of inh A, act A, FS, and E₂. Both FSH alone and LR3 IGF-I alone dose-dependently increased the act A:FS ratio (3-fold; P < 0.005) and act A:inh A ratio (3-fold to 6-fold; P < 0.001), suggesting that both factors selectively raise activin "tone" and that this could be a key requirement for FSH and IGF-induction of follicular E₂ production. This hypothesis was reinforced by the finding that addition of FS, to reduce the act A:FS ratio and sequester secreted activin, markedly suppressed (P < 0.001) FSH (3-fold)-, and LR3 IGF-I (2-fold)-induced E₂ output.

FOOTNOTES

First decision: 10 April 2001.

¹ This work was supported by the Biotechnology and Biological Sciences Research Council of Great Britain (grant 45/SO5760 to P.G.K.).

² Correspondence. FAX: 44 118 931 0180; p.g.knight{at}reading.ac.uk

This article has been cited by other articles:

				N. Forde, M. Mihm, M. J. Canty, A. E. Zielak, P. J. Baker, S. Park, P. Lonergan, G. W. Smith, P. M. Coussens, J. J. Ireland, et al. Differential expression of signal transduction factors in ovarian follicle development: a functional role for betaglycan and FIBP in granulosa cells in cattle Physiol Genomics, April 21, 2008; 33(2): 193 - 204. [Abstract] [Full Text] [PDF]

				C. Kawashima, S. Fukihara, M. Maeda, E. Kaneko, C. A. Montoya, M. Matsui, T. Shimizu, N. Matsunaga, K. Kida, Y.-I. Miyake, et al. Relationship between metabolic hormones and ovulation of dominant follicle during the first follicular wave post-partum in high-producing dairy cows Reproduction, January 1, 2007; 133(1): 155 - 163. [Abstract] [Full Text] [PDF]

				D. C. Woods and A. L. Johnson Phosphatase Activation by Epidermal Growth Factor Family Ligands Regulates Extracellular Regulated Kinase Signaling in Undifferentiated Hen Granulosa Cells Endocrinology, October 1, 2006; 147(10): 4931 - 4940. [Abstract] [Full Text] [PDF]

				M A Beg and O J Ginther Follicle selection in cattle and horses: role of intrafollicular factors. Reproduction, September 1, 2006; 132(3): 365 - 377. [Abstract] [Full Text] [PDF]

				W. Luo and M. C. Wiltbank Distinct Regulation by Steroids of Messenger RNAs for FSHR and CYP19A1 in Bovine Granulosa Cells Biol Reprod, August 1, 2006; 75(2): 217 - 225. [Abstract] [Full Text] [PDF]

				M. Mihm, P.J. Baker, J.L.H. Ireland, G.W. Smith, P.M. Coussens, A.C.O. Evans, and J.J. Ireland Molecular Evidence That Growth of Dominant Follicles Involves a Reduction in Follicle-Stimulating Hormone Dependence and an Increase in Luteinizing Hormone Dependence in Cattle Biol Reprod, June 1, 2006; 74(6): 1051 - 1059. [Abstract] [Full Text] [PDF]

				C. Glister, N. P Groome, and P. G Knight Bovine follicle development is associated with divergent changes in activin-A, inhibin-A and follistatin and the relative abundance of different follistatin isoforms in follicular fluid J. Endocrinol., February 1, 2006; 188(2): 215 - 225. [Abstract] [Full Text] [PDF]

				J Buratini Jr, A B Teixeira, I B Costa, V F Glapinski, M G L Pinto, I C Giometti, C M Barros, M Cao, E S Nicola, and C A Price Expression of fibroblast growth factor-8 and regulation of cognate receptors, fibroblast growth factor receptor-3c and -4, in bovine antral follicles Reproduction, September 1, 2005; 130(3): 343 - 350. [Abstract] [Full Text] [PDF]

				O J Ginther, E L Gastal, M O Gastal, and M A Beg In vivo effects of pregnancy-associated plasma protein-A, activin-A and vascular endothelial growth factor on other follicular-fluid factors during follicle deviation in mares Reproduction, April 1, 2005; 129(4): 489 - 496. [Abstract] [Full Text] [PDF]

				C. Glister, S. L. Richards, and P. G. Knight Bone Morphogenetic Proteins (BMP) -4, -6, and -7 Potently Suppress Basal and Luteinizing Hormone-Induced Androgen Production by Bovine Theca Interna Cells in Primary Culture: Could Ovarian Hyperandrogenic Dysfunction Be Caused by a Defect in Thecal BMP Signaling? Endocrinology, April 1, 2005; 146(4): 1883 - 1892. [Abstract] [Full Text] [PDF]

				I. Demeestere, C. Gervy, J. Centner, F. Devreker, Y. Englert, and A. Delbaere Effect of Insulin-Like Growth Factor-I During Preantral Follicular Culture on Steroidogenesis, In Vitro Oocyte Maturation, and Embryo Development in Mice Biol Reprod, June 1, 2004; 70(6): 1664 - 1669. [Abstract] [Full Text] [PDF]

				O.J. Ginther, E.L. Gastal, M.O. Gastal, and M.A. Beg Critical Role of Insulin-Like Growth Factor System in Follicle Selection and Dominance in Mares Biol Reprod, May 1, 2004; 70(5): 1374 - 1379. [Abstract] [Full Text] [PDF]

				C. Glister, C F. Kemp, and P. G Knight Bone morphogenetic protein (BMP) ligands and receptors in bovine ovarian follicle cells: actions of BMP-4, -6 and -7 on granulosa cells and differential modulation of Smad-1 phosphorylation by follistatin Reproduction, February 1, 2004; 127(2): 239 - 254. [Abstract] [Full Text] [PDF]

				T. Fayad, V. Levesque, J. Sirois, D. W. Silversides, and J. G. Lussier Gene Expression Profiling of Differentially Expressed Genes in Granulosa Cells of Bovine Dominant Follicles Using Suppression Subtractive Hybridization Biol Reprod, February 1, 2004; 70(2): 523 - 533. [Abstract] [Full Text] [PDF]

				O.J. Ginther, D.R. Bergfelt, M.A. Beg, C. Meira, and K. Kot In Vivo Effects of an Intrafollicular Injection of Insulin-Like Growth Factor 1 on the Mechanism of Follicle Deviation in Heifers and Mares Biol Reprod, January 1, 2004; 70(1): 99 - 105. [Abstract] [Full Text] [PDF]

				S. M. Quirk, R. G. Cowan, R. M. Harman, C.-L. Hu, and D. A. Porter Ovarian follicular growth and atresia: The relationship between cell proliferation and survival J Anim Sci, January 1, 2004; 82(13_suppl): E40 - 52. [Abstract] [Full Text] [PDF]

				F. Jimenez-Krassel, M. E. Winn, D. Burns, J. L. H. Ireland, and J. J. Ireland Evidence for a Negative Intrafollicular Role for Inhibin in Regulation of Estradiol Production by Granulosa Cells Endocrinology, May 1, 2003; 144(5): 1876 - 1886. [Abstract] [Full Text] [PDF]

				C. Glister, N. P. Groome, and P. G. Knight Oocyte-Mediated Suppression of Follicle-Stimulating Hormone- and Insulin-Like Growth Factor-Induced Secretion of Steroids and Inhibin-Related Proteins by Bovine Granulosa Cells In Vitro: Possible Role of Transforming Growth Factor {alpha} Biol Reprod, March 1, 2003; 68(3): 758 - 765. [Abstract] [Full Text] [PDF]

				O.J. Ginther, M.A. Beg, K. Kot, C. Meira, and D.R. Bergfelt Associated and Independent Comparisons Between the Two Largest Follicles Preceding Follicle Deviation in Cattle Biol Reprod, February 1, 2003; 68(2): 524 - 529. [Abstract] [Full Text] [PDF]

				O.J. Ginther, C. Meira, M.A. Beg, and D.R. Bergfelt Follicle and Endocrine Dynamics During Experimental Follicle Deviation in Mares Biol Reprod, September 1, 2002; 67(3): 862 - 867. [Abstract] [Full Text] [PDF]

				O.J. Ginther, M.A. Beg, D.R. Bergfelt, and K. Kot Activin A, Estradiol, and Free Insulin-Like Growth Factor I in Follicular Fluid Preceding the Experimental Assumption of Follicle Dominance in Cattle Biol Reprod, July 1, 2002; 67(1): 14 - 19. [Abstract] [Full Text] [PDF]