Biol Reprod 2009 SSR Annual Meeting Abstracts
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Biology of Reproduction 65, 1518-1521 (2001)
© 2001 Society for the Study of Reproduction, Inc.

Regular Article

Mouse Offspring after Microinjection of Heated Spermatozoa

J. Cozzia, F. Monier-Gavellea, N. Lièvrea, M. Bomselb, and J.P. Wolf1,,a

a Laboratoire d'Histologie et de Biologie de la Reproduction, Equipe d'Accueil 2138, UFR Science Médecine Biologie Humaine, Université Paris 13, 93017 Bobigny, France b U 332-INSERM, Institut Cochin de Génétique Moléculaire, 75014 Paris, France

ABSTRACT

The thermostability of the mammalian sperm genome was previously reported, but no live offspring after conception with heated spermatozoa had yet been obtained. In the present study, mouse spermatozoa were heated at 56°C for 30 min and microinjected into mouse oocytes. Fertilization did not occur unless activation was induced by incubation in a calcium-free medium containing strontium. Under these conditions fertilization and cleavage rates were comparable to those obtained after microinjection of control spermatozoa, but the developmental rate to the blastocyst stage was lower. When transferred to foster mothers, embryos derived from heated sperm developed into phenotypically normal offspring, which grew and reproduced normally. In the mouse, heated spermatozoa can therefore support full embryonic development after microinjection into oocytes.

FOOTNOTES

First decision: 24 January 2001.

1 Correspondence: Jean Philippe Wolf, Laboratoire d'Histologie et de Biologie de la Reproduction, UFR Science Médecine Biologie Humaine, 74 Rue Marcel Cachin, 93017 Bobigny, France. FAX: 33 1 48 38 77 77; jean-philippe.wolf{at}jvr.ap-hop-paris.fr




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