HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Reggio, B. C. Articles by Godke, R. A. Search for Related Content PubMed PubMed Citation Articles by Reggio, B. C. Articles by Godke, R. A. Agricola Articles by Reggio, B. C. Articles by Godke, R. A.

Cloned Transgenic Offspring Resulting from Somatic Cell Nuclear Transfer in the Goat: Oocytes Derived from Both Follicle-Stimulating Hormone-Stimulated and Nonstimulated Abattoir-Derived Ovaries¹

^a Department of Animal Science, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803 ^b Genzyme Transgenics Corporation, Framingham, Massachusetts 01701

ABSTRACT

The use of nuclear transfer (NT) techniques to create transgenic offspring capable of producing valuable proteins may have a major impact on the pharmaceutical market. Our objective was to compare the in vivo developmental potential of NT embryos produced from the fusion of transgenic donor cells with cytoplasts prepared from either FSH-stimulated ovaries or nonstimulated abattoir-derived ovaries. Donor cells were prepared from a transgenic fetus carrying the gene for human antithrombin III as a marker and used within four to eight subpassages. Cells were serum deprived for 4 days prior to cytoplast transfer. Oocytes were enucleated by removing the metaphase plate using a DNA stain and epifluorescent illumination. Donor cells were fused to enucleated oocytes by electric pulse and then chemically activated. There was no difference in the number of transferable embryos produced from cytoplasts of FSH-stimulated ovaries or from the fusion of cytoplasts from abattoir ovaries, nor was there a difference in the number of pregnancies established per recipient with either treatment. All pregnancies from both groups culminated in the births of healthy female kids (five total). To our knowledge, this is the first report of cloned goats produced from NT using cytoplasts derived from abattoir ovaries.

FOOTNOTES

First decision: 7 May 2001.

¹ This manuscript was submitted with the approval of the Director of the Louisiana Agricultural Experiment Station as Manuscript 01-11-0277. This study was part of the W-171 Federal Regional Project. This research was supported in part by funds from the Louisiana Agricultural Experiment Station and by a research grant from Genzyme Transgenics, Framingham, MA.

² Correspondence: Robert Godke, Department of Animal Science, Louisiana State University, 105 Francioni Hall, Baton Rouge, LA 70803. FAX: 225 642 0048; rgodke{at}agctr.lsu.edu

This article has been cited by other articles:

				V. Bordignon, R. Keyston, A. Lazaris, A. S. Bilodeau, J. H.F. Pontes, D. Arnold, G. Fecteau, C. Keefer, and L. C. Smith Transgene Expression of Green Fluorescent Protein and Germ Line Transmission in Cloned Calves Derived from In Vitro-Transfected Somatic Cells Biol Reprod, June 1, 2003; 68(6): 2013 - 2023. [Abstract] [Full Text] [PDF]

				J. R. Hill, D. H. Schlafer, P. J. Fisher, and C. J. Davies Abnormal Expression of Trophoblast Major Histocompatibility Complex Class I Antigens in Cloned Bovine Pregnancies Is Associated with a Pronounced Endometrial Lymphocytic Response Biol Reprod, July 1, 2002; 67(1): 55 - 63. [Abstract] [Full Text] [PDF]