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Regular Article |
and ERß1
a Departments of Animal Science and
b Veterinary Biosciences, University of Illinois at Urbana-Champaign, Urbana, Illinois 61802
ABSTRACT
Earlier studies have shown that the efferent ductules (ED) of the male mouse are a target for estrogen. The loss of estrogen receptor (ER) function through either knockout technology (
ERKO mouse) or chemical interference (pure antagonist, ICI 182 780) results in a failure of a major function of the ED, the reabsorption of testicular fluids. The purpose of this study was to test the hypothesis that estrogen controls fluid (water) reabsorption in the ED by modulating ion transporters important for passive water movement through a leaky epithelium such as the ED. Northern blot analysis was used to detect the mRNA levels for key ion transporters in the following experimental groups: 1) wild-type (WT) control for the 14-day experiment, 2) ER knockout (ERKO) control for the 14-day experiment, 3) WT treated with ICI 182 780 (ICI) for 14 days, 4) ERKO treated with ICI for 14 days, 5) WT control for the 35-day experiment, and 6) WT treated with ICI for 35 days. Estrogen differentially modulated the mRNA levels of key ion transporters. ER mediated carbonic anhydrase II mRNA abundance, and there was a decrease in Na+/H+ exchanger 3 mRNA levels in the ERKO that appeared to be a cellular effect and not a direct estrogen effect. The loss of ER control resulted in an increase in mRNA abundance for the catalytic subunit of Na+-K+ ATPase 1, whereas an increase in the mRNA abundance of the Cl-/HCO3- exchanger and the chloride channel cystic fibrosis transmembrane regulator was significantly ERß mediated. Our results indicate for the first time that estrogen acting directly and indirectly through both ER and ERß probably modulates fluid reabsorption in the adult mouse ED by regulating the expression of ion transporters involved in the movement of Na+ and Cl-.
1 This work was supported in part by USDA grant AG9735203-4615 and NIH grant HD-35126.
2 Correspondence: David Bunick, Department of Veterinary Bioscience, University of Illinois, 2001 South Lincoln Ave., Urbana, IL 61802. FAX: 217 244 1652; d-bunick{at}uiuc.edu
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