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Regular Article |
a Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Denmark
Angiotensin (Ang) II may modulate reproductive function in the bovine ovary. Therefore, expression and localization of a local ovarian renin-angiotensin system (RAS) were investigated by elucidating the influence of the estrus cycle, pregnancy, and the presence of follicular cysts. Receptor analysis and autoradiography were used to characterize and localize Ang II receptors. Cyclic variations in the density of ovarian Ang II receptors were found with a higher value in estrus than in diestrus. The density in ovaries with follicular cysts was in the same order of magnitude as in estrus. The Ang II receptor type 2 (AT2) dominated in all three groups. Autoradiography showed that the majority of antral follicles and follicular cysts had intense AT2 receptor binding in the theca externa. Binding was less intense in the theca interna, whereas there was no binding in the granulosa layer. In the corpora lutea, the AT2 receptor was dominant in the capsule and in connective tissue infoldings, whereas no binding was observed in the luteal tissue. The type 1 Ang II receptor (AT1) was dominant in the stroma and showed no cyclic changes. Angiotensin-converting enzyme (ACE) activity was detected in all aspirated follicular fluids and homogenates of ovarian tissue. Autoradiography showed that most of the ACE was localized on endothelial cells. Renin immunoreactivity was found in granulosa and thecal cells of antral follicles and in luteal cells. Furthermore, solitary cells in the stroma, presumably macrophages, displayed intense staining. Our finding of cyclic changes support the concept of an active and regulated RAS in the bovine ovary.
1 This study was partially supported by grants from the Danish Agricultural and Veterinary Research Council, the Nordic Insulin Fund, and the NOVO Fund. Losartan (DuP 753) and PD123319 were gifts from DuPont Merck Pharmaceutical Company, Wilmington, DE, and Parke-Davis, Ann Arbor, MI, respectively.
2 Correspondence: K.H. Schauser, Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, Grønnegaardsvej 7, DK-1870 Frederiksberg C, Denmark. FAX: 45 35282525; ks{at}kvl.dk
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