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Regular Article |
Regulation of the Bovine Corpus Luteum Endothelin System During the Early and Midluteal Phase1
a Department of Biology, West Virginia University, Morgantown, West Virginia 26506-6057
b Agriculture Research, Virginia State University, Petersburg, Virginia 23806
c Division of Animal and Veterinary Science College of Agricultural Forestry and Consumer Science, West Virginia University, Morgantown, West Virginia 26506
Recent evidence in the cow suggests that endothelin-1 (ET-1) plays a role during prostaglandin (PG) F2
-induced luteal regression. We have examined the effects of treatment with PGF2 during the early and midluteal phases on three components of the endothelin system: endothelin-converting enzyme-1 (ECE-1), ET type A receptor (ETA), and ET-1 in the bovine corpus luteum (CL). Cyclic beef cows were injected (0 h) on Day 4 or 10 with either saline or the PGF2 analogue Lutalyse (15 mg). The CL were collected at 2 (n = 11), 10 (n = 23), 24 (n = 15), or 48 h (n = 12) after treatment. The cows in which CL were removed after 10 h comprised of two experimental groups. The first group (n = 11) received one injection; the second group (n = 12) received two injections, one at 0 h and one at 8 h. The cows in which CL were collected after 24 and 48 h received one injection every 8 h. Semiquantitative reverse transcriptase-polymerase chain reaction was used to evaluate the mRNA encoding ECE-1, ETA, and ET-1. The ECE-1 and ETA proteins were evaluated by semiquantitative Western blot analysis. The ET-1 was the most likely component of the endothelin system target for PGF2 regulation during the midluteal phase. The ETA and ECE-1 genes were constitutively expressed in the Day 4 and Day 10 CL. A practical application of this observation is that it may be possible to target the ET-1 gene as a way to manipulate the luteolytic action of PGF2.
1 Supported in part by USDA/CREES award 98-3503-6634 to J.A.F. and Hatch 321 (NE 161) to E.K.I.
2 Correspondence: Jorge A. Flores, Department of Biology, West Virginia University, P.O. Box 6057, Morgantown, WV 26506-6057. FAX: 304 293 6363; jaf5s{at}wvnvm.wvnet.edu
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