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Regular Article |
a Utrecht University, Faculty of Biology, Research Group Comparative Endocrinology, 3584 CH Utrecht, The Netherlands
Male fish produce 11-ketotestosterone as a potent androgen in addition to testosterone. Previous experiments with juvenile African catfish (Clarias gariepinus) showed that 11-ketotestosterone, but not testosterone, stimulated spermatogenesis, whereas testosterone, but not 11-ketotestosterone, accelerated pituitary gonadotroph development. Here, we investigated the effects of combined treatment with these two types of androgens on pituitary gonadotroph and testis development. Immature fish were implanted for 2 wk with silastic pellets containing 11-ketotestosterone, testosterone, 5
-dihydrotestosterone, or estradiol-17ß; cotreatment groups received 11-ketotestosterone in combination with one of the other steroids. Testicular weight and pituitary LH content were higher (two- and fivefold, respectively) in the end control than in the start control group, reflecting the beginning of normal pubertal development. Treatment with testosterone or estradiol-17ß further increased the pituitary LH content four- to sixfold above the end control levels. This stimulatory effect on the pituitary LH content was not modulated by cotreatment with 11-ketotestosterone. However, the stimulatory effect of 11-ketotestosterone on testis growth and spermatogenesis was abolished by cotreatment with testosterone, but not by cotreatment with estradiol-17ß or 5-dihydrotestosterone. Also, normal pubertal testis development was inhibited by prolonged (4 wk) treatment with testosterone. The inhibitory effect of testosterone may involve feedback effects on pituitary FSH and/or on FSH receptors in the testis. It appears that the balanced production of two types of androgens, and the control of their biological activities, are critical to the regulation of pubertal development in male African catfish.
1 J.E.B.C. was supported by Project "PRAXIS XXI" J.N.I.C.T.-Portugal, grant BD/2603/93.
2 Correspondence: R.W. Schulz, Utrecht University, Faculty of Biology, Research Group Comparative Endocrinology, Padualaan 8, 3584 CH Utrecht, The Netherlands. FAX: 31 30 2532837; r.w.schulz{at}bio.uu.nl
3 Current Address: Molecular and Comparative Endocrinology, Universidade do Algarve, Faculdade de Ciências do Mar e Ambiente-CCMar, Campus de Gambelas, P-8000-810 Faro, Portugal.
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