HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Selam, B. Articles by Arici, A. Search for Related Content PubMed PubMed Citation Articles by Selam, B. Articles by Arici, A. Agricola Articles by Selam, B. Articles by Arici, A.

Extracellular Matrix-Dependent Regulation of Fas Ligand Expression in Human Endometrial Stromal Cells

^a Yale University School of Medicine, Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, New Haven, Connecticut 06520

Interaction between endometrial stromal cells and extracellular matrix (ECM) components has a crucial role in the development of endometriosis. Endometrial stromal cells attach to the mesothelial surface of peritoneum by means of integrins during their initial implantation and growth in endometriosis. Similarly, interaction between integrin and the extracellular matrix is also crucial for the remodeling of the endometrium during early pregnancy. We hypothesized that adhesion of endometrial stromal cells to the extracellular matrix could suppress the immunologic reaction to implanting endometrial cells by inducing the expression of Fas ligand (FasL), a mediator of the apoptotic pathway. Western blot analysis of human endometrial stromal cells plated onto fibronectin, laminin, and collagen IV revealed higher levels of FasL protein expression compared with endometrial stromal cells that plated to BSA-coated plates (control). Immunocytochemistry results from endometrial stromal cells plated to extracellular matrix proteins demonstrated a similar up-regulation of FasL expression. Eutopic endometrial stromal cells from women with endometriosis demonstrated higher FasL expression on control plates and those coated with extracellular matrix proteins compared with those from women without endometriosis. Disruption of actin cytoskeleton in endometrial stromal cells by treatment with cytochalasin D blocked the increase of FasL protein expression that occurred in response to adhesion to the extracellular matrix. These results suggest that attachment of endometrial stromal cells during retrograde menstruation to a new environment such as peritoneum with increased expression of laminin, fibronectin, and collagen IV could lead to an increase in FasL expression. Induction of FasL expression by adhesion of endometrial stromal cells to the extracellular matrix may take part in the development of a relative immunotolerance by inducing apoptosis of cytotoxic T lymphocytes, which will allow further development of ectopic implants.

First decision: 1 February 2001.

¹ Correspondence: Aydin Arici, Yale University School of Medicine, Department of Obstetrics and Gynecology, 333 Cedar Street, New Haven, CT 06520-8063. FAX: 203 785 7134; aydin.arici{at}yale.edu

² Current address: IVI-Madrid, C/Santiago de Compostela 88, 28035 Madrid, Spain.

This article has been cited by other articles:

				J. Prast, L. Saleh, H. Husslein, S. Sonderegger, H. Helmer, and M. Knofler Human Chorionic Gonadotropin Stimulates Trophoblast Invasion through Extracellularly Regulated Kinase and AKT Signaling Endocrinology, March 1, 2008; 149(3): 979 - 987. [Abstract] [Full Text] [PDF]

				J. D. Wren, Y. Wu, and S.-W. Guo A system-wide analysis of differentially expressed genes in ectopic and eutopic endometrium Hum. Reprod., August 1, 2007; 22(8): 2093 - 2102. [Abstract] [Full Text] [PDF]

				M. Ulukus, H. Cakmak, and A. Arici The Role of Endometrium in Endometriosis Reproductive Sciences, October 1, 2006; 13(7): 467 - 476. [Abstract] [PDF]

				B. Selam, U. A. Kayisli, G. E. Akbas, M. Basar, and A. Arici Regulation of FAS Ligand Expression by Chemokine Ligand 2 in Human Endometrial Cells Biol Reprod, August 1, 2006; 75(2): 203 - 209. [Abstract] [Full Text] [PDF]

				J. B. Lee, J. E. Lee, J. H. Park, S. J. Kim, M. K. Kim, S. I. Roh, and H. S. Yoon Establishment and Maintenance of Human Embryonic Stem Cell Lines on Human Feeder Cells Derived from Uterine Endometrium under Serum-Free Condition Biol Reprod, January 1, 2005; 72(1): 42 - 49. [Abstract] [Full Text] [PDF]

				J. B. Lee, J. M. Song, J. E. Lee, J. H. Park, S. J. Kim, S. M. Kang, J. N. Kwon, M. K. Kim, S. I. Roh, and H. S. Yoon Available human feeder cells for the maintenance of human embryonic stem cells Reproduction, December 1, 2004; 128(6): 727 - 735. [Abstract] [Full Text] [PDF]

				O. Guzeloglu Kayisli, U. A. Kayisli, G. Luleci, and A. Arici In Vivo and In Vitro Regulation of Akt Activation in Human Endometrial Cells Is Estrogen Dependent Biol Reprod, September 1, 2004; 71(3): 714 - 721. [Abstract] [Full Text] [PDF]

				T. Harada, A. Kaponis, T. Iwabe, F. Taniguchi, G. Makrydimas, N. Sofikitis, M. Paschopoulos, E. Paraskevaidis, and N. Terakawa Apoptosis in human endometrium and endometriosis Hum. Reprod. Update, January 1, 2004; 10(1): 29 - 38. [Abstract] [Full Text] [PDF]

				B. Selam, U. A. Kayisli, J. A. Garcia-Velasco, G. E. Akbas, and A. Arici Regulation of Fas Ligand Expression by IL-8 in Human Endometrium J. Clin. Endocrinol. Metab., August 1, 2002; 87(8): 3921 - 3927. [Abstract] [Full Text] [PDF]