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Regular Article |
a Department of Obstetrics and Gynaecology, Queen Elizabeth Building, Glasgow Royal Infirmary, University of Glasgow, Glasgow G31 2ER, United Kingdom
Each of the proinflammatory cytokines interleukin (IL)-1ß, IL-6, IL-8, and tumor necrosis factor (TNF) 
has been identified in reproductive tissues during labor. The cellular origin of these cytokines is unclear. The aim of this study was to localize these proinflammatory cytokines in myometrium (upper and lower segment), cervix, and fetal membranes at term. Biopsies were taken from women undergoing cesarean section either before or after the onset of labor. Immunohistochemistry was used to localize each of the cytokines IL-1ß, IL-6, IL-8, and TNF. Leukocytes were localized using an antibody to CD45. In myometrium and cervix, immunostaining for IL-1ß was predominantly in leukocytes. In fetal membranes, IL-1ß localized to leukocytes and to the stromal cells of the decidua. In myometrium, IL-6, IL-8, and TNF were restricted to leukocytes, which were present in greater numbers in tissue obtained during labor. In cervix, IL-6, IL-8, and TNF localized to leukocytes and glandular and surface epithelium. IL-8 also localized to cervical stromal cells. In fetal membranes, IL-6 and TNF were expressed by decidual stromal cells, infiltrating leukocytes, and extravillous trophoblasts. In membranes, IL-8 localized to leukocytes in the chorion but was not detected in the amnion. In fetal membranes collected at labor, IL-8 was expressed in decidual stromal cells. Infiltrating leukocytes are a major source of cytokines in uterine tissues during labor.
1 This work was funded in part by grants from the Scottish Hospital Endowments Research Trust (1442) and from TENOVUS Scotland (S99/2).
2 Correspondence: Jane Norman, Department of Obstetrics and Gynaecology, Queen Elizabeth Building, Glasgow Royal Infirmary, University of Glasgow, 10 Alexandra Parade, Glasgow G31 2ER, UK. FAX: 44 141 553 1367; j.e.norman{at}clinmed.gla.ac.uk
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