HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Macháty, Z. Articles by Prather, R. S. Search for Related Content PubMed PubMed Citation Articles by Macháty, Z. Articles by Prather, R. S. Agricola Articles by Macháty, Z. Articles by Prather, R. S.

Capacitative Calcium Entry Mechanism in Porcine Oocytes¹

^a Alexion Pharmaceuticals, Inc., Cheshire, Connecticut 06410 ^b Department of Animal Sciences, University of Missouri, Columbia, Missouri 65211

The presence of the capacitative Ca²⁺ entry mechanism was investigated in porcine oocytes. In vitro-matured oocytes were treated with thapsigargin in Ca²⁺-free medium for 3 h to deplete intracellular calcium stores. After restoring extracellular calcium, a large calcium influx was measured by using the calcium indicator dye fura-2, indicating capacitative Ca²⁺ entry. A similar divalent cation influx could also be detected with the Mn²⁺-quench technique after inositol 1,4,5-triphosphate-induced Ca²⁺ release. In both cases, lanthanum, the Ca²⁺ permeable channel inhibitor, completely blocked the influx caused by store depletion. Heterologous expression of Drosophila trp in porcine oocytes enhanced the thapsigargin-induced Ca²⁺ influx. Polymerase chain reaction cloning using primers that were designed based on mouse and human trp sequences revealed that porcine oocytes contain a trp homologue. As in other cell types, the capacitative Ca²⁺ entry mechanism might help in refilling the intracellular stores after the release of Ca²⁺ from the stores. Further investigation is needed to determine whether the trp channel serves as the capacitative Ca²⁺ entry pathway in porcine oocytes or is simply activated by the endogenous capacitative Ca²⁺ entry mechanism and thus contributes to Ca²⁺ influx.

First decision: 29 August 2001.

¹ This report is based on work supported by the Cooperative State Research, Education and Extension Service, U.S. Department of Agriculture, under agreement 99-35203-7675.

² Correspondence and current address: Zoltán Macháty, Columbus Farming Corporation, P.O. Box 1160, Sherburne, NY 13460. FAX: 607 674 6309; machatyz{at}columbusfarming.com

This article has been cited by other articles:

				F. J. Martin-Romero, J. R. Ortiz-de-Galisteo, J. Lara-Laranjeira, J. A. Dominguez-Arroyo, E. Gonzalez-Carrera, and I. S Alvarez Store-Operated Calcium Entry in Human Oocytes and Sensitivity to Oxidative Stress Biol Reprod, February 1, 2008; 78(2): 307 - 315. [Abstract] [Full Text] [PDF]