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Regular Article |
a Department of Anatomy and Physiology, Kansas State University, Manhattan, Kansas 66506
Epithelial cells were isolated from adult porcine vas deferens and grown in the absence or presence of steroid hormones. Transepithelial resistance (Rte), basal short circuit current (Isc), and the effects of selected ion transport modulators on these parameters were evaluated in modified Ussing chambers at three time points (5–8, 11–14, and 18–22 days postseeding). At the earliest time point, no significant differences were observed. At the middle time point, when compared with Rte in untreated control monolayers, Rte in monolayers exposed to 17ß-estradiol, aldosterone, cortisol, cortisone, prednisolone, prednisone, and dexamethasone was significantly lower; in contrast, Rte in monolayers exposed to testosterone, dihydrotestosterone, or progesterone did not differ from that in control monolayers. Treatments with cortisol, prednisolone, and dexamethasone were associated with an elevated basal Isc that was amiloride sensitive, indicating ongoing Na+ absorption by these monolayers. Rte was increased by amiloride treatment in glucocorticoid-treated monolayers but remained significantly less than that of control monolayers. At the third time point, the postamiloride Rte of glucocorticoid-treated monolayers was not different from that of control monolayers. Responses to ATP, forskolin, bumetanide, and DASU-02 were not affected by steroid treatment at any time point. Taken together, these results suggest that estrogens and corticosteroids can modulate epithelial function in the distal excurrent duct of the adult male reproductive system. At physiological or pharmacological concentrations, these hormones would be expected to modify the luminal environment (both the ionic composition and pH) to which sperm are exposed and thus affect male fertility.
1 Supported by the Cystic Fibrosis Foundation (SCHULT99P0) and the Kansas State University College of Veterinary Medicine Dean's Research Fund. Contribution 02-39-J from the Kansas Agricultural Experiment Station.
2 Correspondence: Bruce D. Schultz, Department of Anatomy and Physiology, Kansas State University, 1600 Denison Ave., Coles Hall 228, Manhattan, KS 66506. FAX: 785 532 4557; bschultz{at}vet.ksu.edu
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